Promoter Screening Facilitates Heterologous Production of Complex Secondary Metabolites in Burkholderiales Strains.

Burkholderiaceae / genetics Burkholderiales / genetics Epothilones / biosynthesis Gene Expression Regulation, Bacterial Genes, Reporter Multigene Family Myxococcales / genetics Peptide Synthases / genetics Polyketide Synthases / genetics Promoter Regions, Genetic / genetics Real-Time Polymerase Chain Reaction
Burkholderiales Schlegelella brevitalea constitutive promoters epothilone secondary metabolites

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
21 02 2020
Historique:
pubmed: 31 1 2020
medline: 12 1 2021
entrez: 31 1 2020
Statut: ppublish

Résumé

Burkholderiales are an emerging source of bioactive secondary metabolites and have the potential to be a robust chassis for metabolites from Gram-negative bacteria. However, only a few constitutive promoters can be utilized in Burkholderiales. Herein, we described the screening of strong constitutive promoters from Burkholderiales strain DSM 7029, and 37 promoters identified from transcriptome sequencing were cloned and characterized using a firefly luciferase reporter and were further verified by qPCR analysis. These promoters were then used to drive a complex 56-kb epothilone BGC from myxobacterium and a 23-kb rhizomide BGC from

Identifiants

DOI: 10.1021/acssynbio.9b00459 PMID: 31999442
pubmed: 31999442
doi: 10.1021/acssynbio.9b00459
doi:

Substances chimiques

Epothilones 0
Polyketide Synthases 79956-01-7
Peptide Synthases EC 6.3.2.-
non-ribosomal peptide synthase EC 6.3.2.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

457-460

Auteurs

Qing Ouyang (Q)

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology , Shandong University , Qingdao , Shandong 266237 , PR China.

Xue Wang (X)

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology , Shandong University , Qingdao , Shandong 266237 , PR China.

Na Zhang (N)

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology , Shandong University , Qingdao , Shandong 266237 , PR China.

Lin Zhong (L)

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology , Shandong University , Qingdao , Shandong 266237 , PR China.

Jiaqi Liu (J)

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology , Shandong University , Qingdao , Shandong 266237 , PR China.

Xiaoming Ding (X)

Collaborative Innovation Center for Genetics and Development, State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences , Fudan University , Shanghai 200433 , PR China.

Youming Zhang (Y)

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology , Shandong University , Qingdao , Shandong 266237 , PR China.

Xiaoying Bian (X)

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology , Shandong University , Qingdao , Shandong 266237 , PR China.
ORCID: 0000-0002-1356-3211

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Bactéries › Proteobacteria › Betaproteobacteria › Burkholderiales › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Composés chimiques organiques › Lactones › Épothilones › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Gènes rapporteurs › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Famille multigénique › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Bactéries › Proteobacteria › Deltaproteobacteria › Myxococcales › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Ligases › Carbon-nitrogen ligases › Amino-acid ligases › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Complexes multienzymatiques › Polyketide synthases › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Régions promotrices (génétique) › Promoter Screening Facilitates Heterologous...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Promoter Screening Facilitates Heterologous...