Molecular identification and functional characterization of a cyanogenic glucosyltransferase from flax (Linum unsitatissimum).
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
07
10
2019
accepted:
30
12
2019
entrez:
6
2
2020
pubmed:
6
2
2020
medline:
23
4
2020
Statut:
epublish
Résumé
Flax seed has become consumers' choice for not only polyunsaturated alpha-linolenic fatty acid but also nutraceuticals such as lignans and soluble fiber. There is, however, a major drawback of flax as a source of functional food since the seeds contain significant level of cyanogenic glucosides. The final step of cyanogenic glucoside biosynthesis is mediated by UDP-glucose dependent glucosyltransferase. To date, no flax cyanogenic glucosyl transferase genes have been reported with verified biochemical functionality. Here we present a study on the identification and enzymatic characterization of a first flax cyanogenic glucosyltransferase, LuCGT1. We show that LuCGT1 was highly active towards both aliphatic and aromatic substrates. The LuCGT1 gene is expressed in leaf tissues as well as in developing seeds, and its expression level was drastically reduced in flax mutant lines low in cyanogenic glucosides. Identification of LuCGT1 provides a molecular handle for developing gene specific markers for targeted breeding of low cyanogenic glucosides in flax.
Identifiants
pubmed: 32023283
doi: 10.1371/journal.pone.0227840
pii: PONE-D-19-28026
pmc: PMC7001965
doi:
Substances chimiques
Nitriles
0
cyanogen
534Q0F66RK
Glucosyltransferases
EC 2.4.1.-
Uridine Diphosphate Glucose
V50K1D7P4Y
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0227840Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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