Functional replacement of isoprenoid pathways in Rhodobacter sphaeroides.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
02
12
2019
revised:
30
01
2020
accepted:
02
03
2020
pubmed:
25
3
2020
medline:
15
5
2021
entrez:
25
3
2020
Statut:
ppublish
Résumé
Advances in synthetic biology and metabolic engineering have proven the potential of introducing metabolic by-passes within cell factories. These pathways can provide a more efficient alternative to endogenous counterparts due to their insensitivity to host's regulatory mechanisms. In this work, we replaced the endogenous essential 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the industrially relevant bacterium Rhodobacter sphaeroides by an orthogonal metabolic route. The native 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway was successfully replaced by a heterologous mevalonate (MVA) pathway from a related bacterium. The functional replacement was confirmed by analysis of the reporter molecule amorpha-4,11-diene after cultivation with [4-
Identifiants
pubmed: 32207882
doi: 10.1111/1751-7915.13562
pmc: PMC7264872
doi:
Substances chimiques
Sesquiterpenes
0
Mevalonic Acid
S5UOB36OCZ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1082-1093Informations de copyright
© 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
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