Functional characterization of the promoter of pearl millet heat shock protein 10 (PgHsp10) in response to abiotic stresses in transgenic tobacco plants.
Chaperonin 10
/ genetics
Cloning, Molecular
Droughts
Gene Expression Regulation, Plant
Pennisetum
/ genetics
Plant Proteins
/ genetics
Plant Structures
/ genetics
Plants, Genetically Modified
Promoter Regions, Genetic
/ genetics
Stress, Physiological
/ genetics
Nicotiana
/ genetics
Transformation, Genetic
Abiotic stress
Chaperonins
GUS histochemical assay
Heat stress
Pearl millet
Pennisetum glaucum
PgHsp10
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Aug 2020
01 Aug 2020
Historique:
received:
18
01
2020
revised:
08
04
2020
accepted:
09
04
2020
pubmed:
16
4
2020
medline:
12
3
2021
entrez:
16
4
2020
Statut:
ppublish
Résumé
In the present study, the promoter region of the pearl millet heat shock protein 10 (PgHsp10) gene was cloned and characterized. The PgHsp10 promoter (PgHsp10pro) sequence region has all the cis-motifs required for tissue and abiotic stress inducibility. The complete PgHsp10pro (PgHsp10PC) region and a series of 5' truncations of PgHsp10 (PgHsp10D1 and PgHsp10D2) and an antisense form of PgHsp10pro (PgHsp10AS) were cloned into a plant expression vector (pMDC164) through gateway cloning. All four constructs were separately transformed into tobacco through Agrobacterium-mediated genetic transformation, and PCR-confirmed transgenic plants progressed to T
Identifiants
pubmed: 32294498
pii: S0141-8130(20)32924-X
doi: 10.1016/j.ijbiomac.2020.04.069
pii:
doi:
Substances chimiques
Chaperonin 10
0
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103-110Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no conflict of interest.