Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers.

Bacteria / metabolism Benchmarking Biosynthetic Pathways Chemical Industry Computer Simulation Fermentation Industrial Microbiology / methods Mandelic Acids / metabolism Metabolic Engineering / methods Stereoisomerism
Biofoundry Biomanufacturing Hydroxymandelic acid Industrial biotechnology Mandelic acid Synthetic biology

Journal

Metabolic engineering
ISSN: 1096-7184
Titre abrégé: Metab Eng
Pays: Belgium
ID NLM: 9815657

Informations de publication

Date de publication:
07 2020
Historique:
received: 22 01 2020
revised: 09 04 2020
accepted: 16 04 2020
pubmed: 27 4 2020
medline: 29 5 2021
entrez: 27 4 2020
Statut: ppublish

Résumé

Bio-based production of industrial chemicals using synthetic biology can provide alternative green routes from renewable resources, allowing for cleaner production processes. To efficiently produce chemicals on-demand through microbial strain engineering, biomanufacturing foundries have developed automated pipelines that are largely compound agnostic in their time to delivery. Here we benchmark the capabilities of a biomanufacturing pipeline to enable rapid prototyping of microbial cell factories for the production of chemically diverse industrially relevant material building blocks. Over 85 days the pipeline was able to produce 17 potential material monomers and key intermediates by combining 160 genetic parts into 115 unique biosynthetic pathways. To explore the scale-up potential of our prototype production strains, we optimized the enantioselective production of mandelic acid and hydroxymandelic acid, achieving gram-scale production in fed-batch fermenters. The high success rate in the rapid design and prototyping of microbially-produced material building blocks reveals the potential role of biofoundries in leading the transition to sustainable materials production.

Identifiants

DOI: 10.1016/j.ymben.2020.04.008 PMID: 32335188 PMC: PMC7225752
pubmed: 32335188
pii: S1096-7176(20)30077-X
doi: 10.1016/j.ymben.2020.04.008
pmc: PMC7225752
pii:
doi:

Substances chimiques

Mandelic Acids 0
mandelic acid NH496X0UJX

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

168-182

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M017702/1
Pays : United Kingdom

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declarations of competing interest None.

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Auteurs

Christopher J Robinson (CJ)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Christopher.Robinson@manchester.ac.uk.

Pablo Carbonell (P)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: pablo.carbonell@manchester.ac.uk.

Adrian J Jervis (AJ)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Adrian.Jervis@manchester.ac.uk.

Cunyu Yan (C)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: cunyu.yan@manchester.ac.uk.

Katherine A Hollywood (KA)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: katherine.hollywood@manchester.ac.uk.

Mark S Dunstan (MS)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Mark.Dunstan@manchester.ac.uk.

Andrew Currin (A)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: andrew.currin@manchester.ac.uk.

Neil Swainston (N)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Neil.Swainston@manchester.ac.uk.

Reynard Spiess (R)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Reynard.Spiess@manchester.ac.uk.

Sandra Taylor (S)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Sandra.Taylor@manchester.ac.uk.

Paul Mulherin (P)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: paul.mulherin@manchester.ac.uk.

Steven Parker (S)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: steven.parker@manchester.ac.uk.

William Rowe (W)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: William.rowe@manchester.ac.uk.

Nicholas E Matthews (NE)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Manchester Institute of Innovation Research, Alliance Manchester Business School, The University of Manchester, Manchester, M15 6PB, UK. Electronic address: n.matthews@manchester.ac.uk.

Kirk J Malone (KJ)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Kirk.Malone@manchester.ac.uk.

Rosalind Le Feuvre (R)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: r.a.le-feuvre@manchester.ac.uk.

Philip Shapira (P)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Manchester Institute of Innovation Research, Alliance Manchester Business School, The University of Manchester, Manchester, M15 6PB, UK. Electronic address: PShapira@manchester.ac.uk.

Perdita Barran (P)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: perdita.barran@manchester.ac.uk.

Nicholas J Turner (NJ)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Nicholas.Turner@manchester.ac.uk.

Jason Micklefield (J)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: jason.micklefield@manchester.ac.uk.

Rainer Breitling (R)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Rainer.Breitling@manchester.ac.uk.

Eriko Takano (E)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Eriko.Takano@manchester.ac.uk.

Nigel S Scrutton (NS)

Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Nigel.Scrutton@manchester.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Rapid prototyping of microbial production...
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questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Voies de biosynthèse › Rapid prototyping of microbial production...
questionsmedicales.fr › Technologie, industrie et agriculture › Industrie › Secteur secondaire › Industrie chimique › Rapid prototyping of microbial production...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Rapid prototyping of microbial production...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › Rapid prototyping of microbial production...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Biotechnologie › Microbiologie industrielle › Rapid prototyping of microbial production...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Hydroxyacides › Acides mandéliques › Rapid prototyping of microbial production...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › Rapid prototyping of microbial production...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes de chimie organique › Isomérie › Stéréoisomérie › Rapid prototyping of microbial production...