Exploring novel bacterial terpene synthases.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
01
12
2019
accepted:
09
04
2020
entrez:
1
5
2020
pubmed:
1
5
2020
medline:
21
7
2020
Statut:
epublish
Résumé
Terpenes are the largest class of natural products with extensive structural diversity and are widely used as pharmaceuticals, herbicides, flavourings, fragrances, and biofuels. While they have mostly been isolated from plants and fungi, the availability and analysis of bacterial genome sequence data indicates that bacteria also possess many putative terpene synthase genes. In this study, we further explore this potential for terpene synthase activity in bacteria. Twenty two potential class I terpene synthase genes (TSs) were selected to represent the full sequence diversity of bacterial synthase candidates and recombinantly expressed in E. coli. Terpene synthase activity was detected for 15 of these enzymes, and included mono-, sesqui- and diterpene synthase activities. A number of confirmed sesquiterpene synthases also exhibited promiscuous monoterpene synthase activity, suggesting that bacteria are potentially a richer source of monoterpene synthase activity then previously assumed. Several terpenoid products not previously detected in bacteria were identified, including aromandendrene, acora-3,7(14)-diene and longiborneol. Overall, we have identified promiscuous terpene synthases in bacteria and demonstrated that terpene synthases with substrate promiscuity are widely distributed in nature, forming a rich resource for engineering terpene biosynthetic pathways for biotechnology.
Identifiants
pubmed: 32353014
doi: 10.1371/journal.pone.0232220
pii: PONE-D-19-33243
pmc: PMC7192455
doi:
Substances chimiques
Terpenes
0
Alkyl and Aryl Transferases
EC 2.5.-
terpene synthase
EC 2.5.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0232220Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L027593/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M000354/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M017702/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M000354/1
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
J Antibiot (Tokyo). 2015 Jun;68(6):385-94
pubmed: 25605043
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Biochemistry. 2015 Dec 8;54(48):7142-55
pubmed: 26598179
Nat Chem Biol. 2007 Nov;3(11):711-5
pubmed: 17873868
Org Lett. 2019 Apr 5;21(7):2426-2429
pubmed: 30859837
Biochemistry. 1994 May 17;33(19):5846-57
pubmed: 8180213
Arch Microbiol. 2002 Dec;178(6):484-92
pubmed: 12420170
Org Biomol Chem. 2016 Apr 7;14(13):3385-93
pubmed: 26947062
Nat Commun. 2011 Sep 27;2:483
pubmed: 21952217
Stand Genomic Sci. 2011 Feb 20;4(1):13-22
pubmed: 21475583
Stand Genomic Sci. 2014 Mar 29;9(3):1062-75
pubmed: 25197483
Chembiochem. 2014 Apr 14;15(6):810-4
pubmed: 24573945
Angew Chem Int Ed Engl. 2019 Jan 8;58(2):452-455
pubmed: 30426646
Angew Chem Int Ed Engl. 2017 Dec 18;56(51):16385-16389
pubmed: 29125678
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W204-12
pubmed: 23737449
Appl Environ Microbiol. 2010 Jan;76(1):136-41
pubmed: 19880637
ACS Catal. 2018 Mar 2;8(3):2012-2020
pubmed: 29750129
Tetrahedron Lett. 1969 Jul;(32):2737-40
pubmed: 5808206
FEBS J. 2017 Jun;284(11):1700-1711
pubmed: 28371548
Science. 1977 May 6;196(4290):680-1
pubmed: 558651
ChemistrySelect. 2016 Jun 21;1(9):1893-1896
pubmed: 29756025
Nat Prod Rep. 2018 Dec 12;35(12):1330-1346
pubmed: 29855001
Nat Chem Biol. 2015 Sep;11(9):625-31
pubmed: 26284661
Metab Eng. 2005 Jan;7(1):18-26
pubmed: 15721807
Phytomedicine. 2010 Nov;17(13):1061-6
pubmed: 20727725
Beilstein J Org Chem. 2017 Aug 23;13:1770-1780
pubmed: 28904620
Beilstein J Org Chem. 2016 Aug 15;12:1839-1850
pubmed: 27829890
Angew Chem Int Ed Engl. 2015 May 11;54(20):6041-5
pubmed: 25809275
Tetrahedron Lett. 1970 Dec;(56):4901-4
pubmed: 5500064
Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14711-5
pubmed: 22908266
Plant Physiol. 2004 Aug;135(4):1908-27
pubmed: 15310829
Chembiochem. 2014 Jan 24;15(2):213-6
pubmed: 24399794
J Antibiot (Tokyo). 1969 Oct;22(10):508-9
pubmed: 5350510
Nat Prod Rep. 2016 Jan;33(1):87-110
pubmed: 26563452
Front Plant Sci. 2017 Aug 24;8:1481
pubmed: 28883829
ACS Catal. 2017 Sep 1;7(9):6268-6282
pubmed: 28966840
Proc Natl Acad Sci U S A. 2015 Jan 20;112(3):857-62
pubmed: 25535391
Metab Eng. 2013 Sep;19:33-41
pubmed: 23727191
Chembiochem. 2011 Sep 5;12(13):1988-91
pubmed: 21726035
Bioinformatics. 2007 Jan 1;23(1):127-8
pubmed: 17050570
Chembiochem. 2019 Mar 1;20(5):677-682
pubmed: 30484946
Chem Rev. 2017 Sep 13;117(17):11570-11648
pubmed: 28841019
J Biochem. 1990 Dec;108(6):995-1000
pubmed: 2089044
Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1881-5
pubmed: 17684275
Front Microbiol. 2016 Dec 21;7:2046
pubmed: 28066367
PLoS Comput Biol. 2016 Aug 12;12(8):e1005053
pubmed: 27517297
Front Plant Sci. 2019 Oct 01;10:1166
pubmed: 31632418
Beilstein J Org Chem. 2019 Nov 29;15:2889-2906
pubmed: 31839835
Antibiot Annu. 1956-1957;:672-5
pubmed: 13425447
Chembiochem. 2011 Nov 4;12(16):2403-7
pubmed: 21910204
Acc Chem Res. 2012 Mar 20;45(3):463-72
pubmed: 22039990
Angew Chem Int Ed Engl. 2013 Feb 4;52(6):1810-2
pubmed: 23307484
Proc Natl Acad Sci U S A. 2008 May 27;105(21):7422-7
pubmed: 18492804
Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12328-12333
pubmed: 27791023
J Bacteriol. 2008 Sep;190(18):6084-96
pubmed: 18658271
Nat Biotechnol. 2003 Jul;21(7):796-802
pubmed: 12778056
Nature. 2013 Apr 25;496(7446):528-32
pubmed: 23575629
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Proc Natl Acad Sci U S A. 2015 May 5;112(18):5661-6
pubmed: 25901324
Appl Microbiol. 1965 Nov;13(6):935-8
pubmed: 5866039