Single cell transcriptomics identifies stem cell-derived graft composition in a model of Parkinson's disease.
Animals
Brain
/ metabolism
Cell Differentiation
Corpus Striatum
Disease Models, Animal
Dopamine
/ metabolism
Dopaminergic Neurons
/ cytology
Embryonic Stem Cells
/ cytology
Female
Graft Survival
Humans
Multigene Family
Parkinson Disease
/ therapy
RNA-Seq
Rats
Rats, Nude
Regeneration
Single-Cell Analysis
Stem Cell Transplantation
Stem Cells
/ cytology
Transcriptome
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
15 05 2020
15 05 2020
Historique:
received:
31
10
2019
accepted:
17
04
2020
entrez:
17
5
2020
pubmed:
18
5
2020
medline:
25
8
2020
Statut:
epublish
Résumé
Cell replacement is a long-standing and realistic goal for the treatment of Parkinson's disease (PD). Cells for transplantation can be obtained from fetal brain tissue or from stem cells. However, after transplantation, dopamine (DA) neurons are seen to be a minor component of grafts, and it has remained difficult to determine the identity of other cell types. Here, we report analysis by single-cell RNA sequencing (scRNA-seq) combined with comprehensive histological analyses to characterize intracerebral grafts from human embryonic stem cells (hESCs) and fetal tissue after functional maturation in a pre-clinical rat PD model. We show that neurons and astrocytes are major components in both fetal and stem cell-derived grafts. Additionally, we identify a cell type closely resembling a class of recently identified perivascular-like cells in stem cell-derived grafts. Thus, this study uncovers previously unknown cellular diversity in a clinically relevant cell replacement PD model.
Identifiants
pubmed: 32415072
doi: 10.1038/s41467-020-16225-5
pii: 10.1038/s41467-020-16225-5
pmc: PMC7229159
doi:
Substances chimiques
Dopamine
VTD58H1Z2X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2434Commentaires et corrections
Type : ErratumIn
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