Fumonisin B1-Induced Changes in Cotton Fiber Elongation Revealed by Sphingolipidomics and Proteomics.
Chromatography, Liquid
Cotton Fiber
Fumonisins
/ pharmacology
Gene Expression Regulation, Plant
/ drug effects
Gossypium
/ drug effects
Metabolic Networks and Pathways
Ovule
/ drug effects
Phenylpropionates
/ metabolism
Plant Development
/ drug effects
Plant Proteins
/ genetics
Proteomics
Sphingolipids
/ antagonists & inhibitors
Tandem Mass Spectrometry
cotton
fiber elongation
fumonisin B1
proteomic
sphingolipid
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
31 08 2020
31 08 2020
Historique:
received:
13
08
2020
revised:
27
08
2020
accepted:
28
08
2020
entrez:
4
9
2020
pubmed:
4
9
2020
medline:
8
9
2021
Statut:
epublish
Résumé
Sphingolipids are essential biomolecules and membrane components, but their regulatory role in cotton fiber development is poorly understood. Here, we found that fumonisin B1 (FB1)-a sphingolipid synthesis inhibitor-could block fiber elongation severely. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), we detected 95 sphingolipids that were altered by FB1 treatment; of these, 29 (mainly simple sphingolipids) were significantly increased, while 33 (mostly complex sphingolipids) were significantly decreased. A quantitative analysis of the global proteome, using an integrated quantitative approach with tandem mass tag (TMT) labeling and LC-MS/MS, indicated the upregulation of 633 and the downregulation of 672 proteins after FB1 treatment. Most differentially expressed proteins (DEPs) were involved in processes related to phenylpropanoid and flavonoid biosynthesis. In addition, up to 20 peroxidases (POD) were found to be upregulated, and POD activity was also increased by the inhibitor. To our knowledge, this is the first report on the effects of FB1 treatment on cotton fiber and ovule sphingolipidomics and proteomics. Our findings provide target metabolites and biological pathways for cotton fiber improvement.
Identifiants
pubmed: 32878249
pii: biom10091258
doi: 10.3390/biom10091258
pmc: PMC7564794
pii:
doi:
Substances chimiques
Fumonisins
0
Phenylpropionates
0
Plant Proteins
0
Sphingolipids
0
fumonisin B1
3ZZM97XZ32
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 31971984 and 31571722
Pays : International
Organisme : Funds for Creative Research Groups of China
ID : 31621005
Pays : International
Organisme : National Transgenic Major Project of China
ID : No. 2018ZX0800921B
Pays : International
Organisme : State Key Laboratory of Cotton Biology Open Fund
ID : CB2020A05
Pays : International
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