Employing lytic phage-mediated horizontal gene transfer in Lactococcus lactis.

Bacteriophages / genetics Cheese / microbiology Dairy Products / microbiology Food Microbiology / methods Gene Transfer Techniques Lactococcus lactis / genetics Plasmids / genetics

Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2020
Historique:
received: 09 12 2019
accepted: 27 08 2020
entrez: 14 9 2020
pubmed: 15 9 2020
medline: 5 11 2020
Statut: epublish

Résumé

Lactococcus lactis is a lactic acid bacterium widely used as a starter culture in the manufacture of dairy products, especially a wide variety of cheeses. Improved industrial strains would help to manufacture better food products that can meet the industry's and consumer's demands with respect to e.g. quality, taste, texture and shelf life. Bacteriophage infection of L. lactis starter cultures represents one of the main causes of fermentation failure and consequent economic losses for the dairy industry. In this study, however, we aim at employing bacteriophages for beneficial purposes. We developed an experimental setup to assess whether phage-mediated horizontal gene transfer could be used to enhance the genetic characteristics of L. lactis strains in accordance with the European law regarding the use of genetically modified organisms (GMOs) in the food industry. Although we could not show the transfer of chromosomal DNA we did successfully transduce two dissimilar plasmids from L. lactis strain MG1363 to one of its derivatives employing three different lactococcal bacteriophages.

Identifiants

DOI: 10.1371/journal.pone.0238988 PMID: 32925946 PMC: PMC7489543
pubmed: 32925946
doi: 10.1371/journal.pone.0238988
pii: PONE-D-19-34001
pmc: PMC7489543
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0238988

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

Références

Appl Environ Microbiol. 2000 Mar;66(3):987-94
pubmed: 10698762
Appl Environ Microbiol. 2006 Jun;72(6):4338-46
pubmed: 16751549
Curr Opin Biotechnol. 2019 Apr;56:61-68
pubmed: 30317145
Mol Gen Genet. 1988 May;212(2):386-9
pubmed: 3405204
Appl Environ Microbiol. 2019 Sep 17;85(19):
pubmed: 31350317
Microb Cell Fact. 2014 Aug 29;13 Suppl 1:S5
pubmed: 25186244
FEMS Microbiol Rev. 2005 Aug;29(3):591-610
pubmed: 15935512
Nucleic Acids Res. 2019 Jul 2;47(W1):W636-W641
pubmed: 30976793
Appl Environ Microbiol. 1995 Nov;61(11):4089-98
pubmed: 8526523
Cell. 2011 Feb 18;144(4):590-600
pubmed: 21335240
Appl Environ Microbiol. 1993 Jun;59(6):1966-8
pubmed: 16348973
Appl Environ Microbiol. 2013 Oct;79(20):6481-90
pubmed: 23956387
Sci Rep. 2015 Feb 10;5:8365
pubmed: 25666585
J Bacteriol. 2008 Apr;190(8):3083-7
pubmed: 18263725
J Bacteriol. 1992 Sep;174(17):5633-8
pubmed: 1324906
Food Microbiol. 2007 Feb;24(1):75-81
pubmed: 16943097
J Bacteriol. 1983 Apr;154(1):1-9
pubmed: 6403500
Adv Appl Microbiol. 2014;89:135-83
pubmed: 25131402
Biotechnol J. 2007 Apr;2(4):426-34
pubmed: 17407210
Appl Environ Microbiol. 2014 Jun;80(11):3508-17
pubmed: 24682298
Appl Environ Microbiol. 1989 Dec;55(12):3119-23
pubmed: 16348073
Appl Environ Microbiol. 1996 Oct;62(10):3662-7
pubmed: 8837421
Appl Environ Microbiol. 2012 Aug;78(15):5417-23
pubmed: 22610425
Sci Rep. 2017 Aug 15;7(1):8292
pubmed: 28811656
Appl Environ Microbiol. 1991 Sep;57(9):2568-75
pubmed: 1768129
Front Microbiol. 2014 Jan 24;5:7
pubmed: 24478767
Appl Environ Microbiol. 1981 Nov;42(5):897-903
pubmed: 16345892
Appl Environ Microbiol. 1990 Sep;56(9):2726-35
pubmed: 16348281
Curr Opin Microbiol. 2017 Aug;38:v-vii
pubmed: 29173837
Bioinformatics. 2015 Sep 1;31(17):2867-9
pubmed: 25910699
Science. 2013 Feb 22;339(6122):883
pubmed: 23430619
Microbiology (Reading). 2012 Dec;158(Pt 12):2936-2945
pubmed: 23023974
FEMS Microbiol Lett. 2002 Oct 29;216(1):85-90
pubmed: 12423757
J Gen Microbiol. 1992 May;138(5):945-50
pubmed: 1645131
Mol Microbiol. 2003 Jul;49(2):277-300
pubmed: 12886937
Bacteriophage. 2011 Jan;1(1):31-45
pubmed: 21687533
Annu Rev Microbiol. 1989;43:267-92
pubmed: 2679356
J Bacteriol. 1973 Sep;115(3):810-5
pubmed: 4199514
Bioinformatics. 2015 Feb 15;31(4):587-9
pubmed: 25338718
Microb Cell Fact. 2017 Apr 4;16(1):55
pubmed: 28376880
J Appl Microbiol. 1997 Jul;83(1):85-90
pubmed: 9246774
J Appl Microbiol. 2001 Jan;90(1):59-67
pubmed: 11155123

Auteurs

Barbara Marcelli (B)

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

Harma Karsens (H)

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

Mark Nijland (M)

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

Ruben Oudshoorn (R)

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

Oscar P Kuipers (OP)

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

Jan Kok (J)

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

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Classifications MeSH

questionsmedicales.fr › Virus › Bactériophages › Employing lytic phage-mediated horizontal gene...
questionsmedicales.fr › Aliments et boissons › Aliments › Produits laitiers › Produits laitiers de culture › Fromage › Employing lytic phage-mediated horizontal gene...
questionsmedicales.fr › Aliments et boissons › Aliments › Produits laitiers › Employing lytic phage-mediated horizontal gene...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Qualité alimentaire › Sécurité des aliments › Contamination des aliments › Microbiologie alimentaire › Employing lytic phage-mediated horizontal gene...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de transfert de gènes › Employing lytic phage-mediated horizontal gene...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Employing lytic phage-mediated horizontal gene...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › Employing lytic phage-mediated horizontal gene...