High-throughput Minitaturized RNA-Seq Library Preparation.
Sequencing, automation, Illumina
Journal
Journal of biomolecular techniques : JBT
ISSN: 1943-4731
Titre abrégé: J Biomol Tech
Pays: United States
ID NLM: 100888641
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
pubmed:
27
10
2020
medline:
10
8
2021
entrez:
26
10
2020
Statut:
ppublish
Résumé
Advances in next-generation sequencing technologies have allowed RNA sequencing to become an increasingly time efficient, cost-effective, and accessible tool for genomic research. We present here an automated and miniaturized workflow for RNA library preparation that minimizes reagent usage and processing time required per sample to generate Illumina compatible libraries for sequencing. The reduced-volume libraries show similar behavior to full-scale libraries with comparable numbers of genes detected and reproducible clustering of samples.
Identifiants
pubmed: 33100919
doi: 10.7171/jbt.20-3104-004
pii: JBT_2020-3104-004
pmc: PMC7566612
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
151-156Subventions
Organisme : NCI NIH HHS
ID : P30 CA014051
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES002109
Pays : United States
Informations de copyright
© Association of Biomolecular Resource Facilities.
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