Heterologous Expression of Cryptomaldamide in a Cyanobacterial Host.
Anabaena
/ metabolism
Biological Products
/ metabolism
Chromatography, High Pressure Liquid
Clustered Regularly Interspaced Short Palindromic Repeats
/ genetics
Gene Editing
/ methods
Multigene Family
Oligopeptides
/ analysis
Peptide Synthases
/ genetics
Plasmids
/ genetics
Polyketide Synthases
/ genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
cryptomaldamide
cyanobacteria
heterologous expression
natural products
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
18 12 2020
18 12 2020
Historique:
pubmed:
13
11
2020
medline:
2
6
2021
entrez:
12
11
2020
Statut:
ppublish
Résumé
Filamentous marine cyanobacteria make a variety of bioactive molecules that are produced by polyketide synthases, nonribosomal peptide synthetases, and hybrid pathways that are encoded by large biosynthetic gene clusters. These cyanobacterial natural products represent potential drug leads; however, thorough pharmacological investigations have been impeded by the limited quantity of compound that is typically available from the native organisms. Additionally, investigations of the biosynthetic gene clusters and enzymatic pathways have been difficult due to the inability to conduct genetic manipulations in the native producers. Here we report a set of genetic tools for the heterologous expression of biosynthetic gene clusters in the cyanobacteria
Identifiants
pubmed: 33180461
doi: 10.1021/acssynbio.0c00431
pmc: PMC8514081
mid: NIHMS1743291
doi:
Substances chimiques
Biological Products
0
Oligopeptides
0
cryptomaldamide
0
Polyketide Synthases
79956-01-7
Peptide Synthases
EC 6.3.2.-
non-ribosomal peptide synthase
EC 6.3.2.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3364-3376Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM118815
Pays : United States
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