Characterization of a novel genomovirus in the phytopathogenic fungus Botrytis cinerea.


Journal

Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674

Informations de publication

Date de publication:
15 01 2021
Historique:
received: 21 05 2020
revised: 31 10 2020
accepted: 12 11 2020
pubmed: 3 12 2020
medline: 4 1 2022
entrez: 2 12 2020
Statut: ppublish

Résumé

This study characterized a single-stranded circular DNA virus in Botrytis cinerea-namely, Botrytis cinerea genomovirus 1 (BcGV1). The genome of BcGV1 was 1710 nucleotides (nts) long, possessing two ORFs, encoding a putative replication initiation protein (Rep) and a hypothetical protein. The Rep contained seven conserved motifs. The two ORFs were separated by two intergenic regions; the large intergenic region (LIR) contained 259 nts while the small intergenic region (SIR) contained 95 nts. A nonanucleotide, TAACAGTAC, in the LIR was predicted to be associated with the initiation of viral replication. Based on the phylogenetic tree constructed by Reps, BcGV1 belongs to the family Genomoviridae, forming an independent branch, indicating that BcGV1 may belong to a new genus. BcGV1 could be detected in 6.7% of tested B. cinerea strains, suggesting that BcGV1 may be widely distributed in the Chinese B. cinerea population.

Identifiants

pubmed: 33264653
pii: S0042-6822(20)30233-6
doi: 10.1016/j.virol.2020.11.007
pii:
doi:

Substances chimiques

Viral Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

111-116

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Fangmin Hao (F)

The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Institute of Vegetables and the Key Lab of Cucurbitaceous Vegetables Breeding in Ningbo City, Ningbo Academy of Agricultural Sciences, Ningbo, 315040, PR China.

Mingde Wu (M)

The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. Electronic address: mingde@mail.hzau.edu.cn.

Guoqing Li (G)

The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

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Classifications MeSH