Occurrence of 'gang of five' Shiga toxin-producing Escherichia coli serogroups on Belgian dairy cattle farms by overshoe sampling.
Adhesins, Bacterial
/ genetics
Animals
Belgium
Cattle
Escherichia coli Infections
/ veterinary
Escherichia coli Proteins
/ genetics
Farms
Feces
/ microbiology
Food Microbiology
Foodborne Diseases
/ microbiology
Immunomagnetic Separation
Serogroup
Shiga Toxin
/ genetics
Shiga Toxin 2
/ genetics
Shiga-Toxigenic Escherichia coli
/ classification
Virulence
Shiga toxin-producing Escherichia coli
cattle
cultural isolation
overshoes
polymerase chain reaction
virulence genes
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
23
09
2020
revised:
08
11
2020
accepted:
30
11
2020
pubmed:
6
12
2020
medline:
20
4
2021
entrez:
5
12
2020
Statut:
ppublish
Résumé
Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens responsible for global outbreaks. This study was conducted to investigate the occurrence of 'gang of five' STEC serogroups (O26, O103, O111, O145, O157) on Belgian dairy cattle farms by overshoe (OVS) sampling, and to evaluate the presence of virulence genes in the obtained isolates. A total of 88 OVS, collected from the pen beddings of 19 Belgian dairy cattle farms, were selectively enriched in mTSBn, followed by immunomagnetic separation and plating onto CT-SMAC for O157 STEC isolation, as well as in Brila broth, followed by a selective acid treatment and plating onto CHROMagar
Substances chimiques
Adhesins, Bacterial
0
Escherichia coli Proteins
0
Shiga Toxin 2
0
eaeA protein, E coli
147094-99-3
Shiga Toxin
75757-64-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
415-419Subventions
Organisme : Belgian Federal Public Service of Health, Food Chain Safety and Environment
ID : RT16/01 VTEFFECT
Informations de copyright
© 2020 The Society for Applied Microbiology.
Références
Berry, E.D. and Miller, D.N. (2005) Cattle feedlot soil moisture and manure content: II. Impact on Escherichia coli O157. J Environ Qual 34, 656-663.
Beutin, L. (2006) Emerging enterohaemorrhagic Escherichia coli, causes and effects of the rise of a human pathogen. J Vet Med Ser B 53, 299-305.
Browne, A.S., Midwinter, A.C., Withers, H., Cookson, A.L., Biggs, P.J., Marshall, J.C., Benschop, J., Hathaway, S. et al. (2018) Molecular epidemiology of shiga toxin-producing Escherichia coli (STEC) on New Zealand dairy farms: application of a culture-independent assay and whole-genome sequencing. Appl Environ Microbiol 84, e00481-18.
Cobbaut, K., Berkvens, D., Houf, K., De Deken, R. and De Zutter, L. (2009) Escherichia coli O157 prevalence in different cattle farm types and identification of potential risk factors. J Food Prot 72, 1848-1853.
Cobbaut, K., Houf, K., Douidah, L., Van Hende, J. and De Zutter, L. (2008) Alternative sampling to establish the Escherichia coli O157 status on beef cattle farms. Vet Microbiol 132, 205-210.
Cobbold, R. and Desmarchelier, P. (2000) A longitudinal study of Shiga-toxigenic Escherichia coli (STEC) prevalence in three Australian dairy herds. Vet Microbiol 71, 125-137.
European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA and ECDC) (2019) The European Union One Health 2018 Zoonoses Report. EFSA J 17, e05926.
FAO and WHO. (2019) Attributing Illness Caused by Shiga Toxin-Producing Escherichia coli (STEC) to Specific Foods. Microbiological Risk Assessment Series no. 32. Rome: FAO & WHO.
Fedio, W.M., Jinneman, K.C., Yoshitomi, K.J., Zapata, R. and Weagant, S.D. (2012) Efficacy of a post enrichment acid treatment for isolation of Escherichia coli O157:H7 from alfalfa sprouts. Food Microbiol 30, 83-90.
Girardeau, J.P., Dalmasso, A., Bertin, Y., Ducrot, C., Bord, S., Livrelli, V., Vernozy-Rozand, C. and Martin, C. (2005) Association of virulence genotype with phylogenetic background in comparison to different seropathotypes of Shiga toxin-producing Escherichia coli isolates. J Clin Microbiol 43, 6098-6107.
Johnson, K.E., Thorpe, C.M. and Sears, C.L. (2006) The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clin Infect Dis Off Publ Infect Dis Soc Am 43, 1587-1595.
Koutsoumanis, K., Allende, A., Alvarez-Ordóñez, A., Bover-Cid, S., Chemaly, M., Davies, R., Cesare, A.D., Herman, L. et al. (2020) Pathogenicity assessment of Shiga toxin-producing Escherichia coli (STEC) and the public health risk posed by contamination of food with STEC. EFSA J 18, e05967.
Mathusa, E.C., Chen, Y., Enache, E. and Hontz, L. (2010) Non-O157 Shiga toxin-producing Escherichia coli in foods. J Food Prot 73, 1721-1736.
Mellor, G.E., Fegan, N., Duffy, L.L., McMILLAN, K.E., Jordan, D. and Barlow, R.S. (2016) National survey of shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157 in Australian Beef Cattle Feces. J Food Prot 79, 1868-1874.
Possé, B., De Zutter, L., Heyndrickx, M. and Herman, L. (2007) Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli. Res Microbiol 158, 591-599.
Robinson, S.E., Wright, E.J., Hart, C.A., Bennett, M. and French, N.P. (2004) Intermittent and persistent shedding of Escherichia coli O157 in cohorts of naturally infected calves. J Appl Microbiol 97, 1045-1053.
Verhaegen, B., Van Damme, I., Heyndrickx, M., Botteldoorn, N., Elhadidy, M., Verstraete, K., Dierick, K., Denayer, S. et al. (2016) Evaluation of detection methods for non-O157 Shiga toxin-producing Escherichia coli from food. Int J Food Microbiol 219, 64-70.
Wang, G., Zhao, T. and Doyle, M.P. (1996) Fate of enterohemorrhagic Escherichia coli O157:H7 in bovine feces. Appl Environ Microbiol 62, 2567-2570.
Zhao, L., Tyler, P.J., Starnes, J., Bratcher, C.L., Rankins, D., McCaskey, T.A. and Wang, L. (2013) Correlation analysis of Shiga toxin-producing Escherichia coli shedding and faecal bacterial composition in beef cattle. J Appl Microbiol 115, 591-603.