Comparative Genome Analysis Reveals Cyanidiococcus gen. nov., A New Extremophilic Red Algal Genus Sister to Cyanidioschyzon (Cyanidioschyzonaceae, Rhodophyta).
Cyanidiococcus gen. nov.
Cyanidiococcus yangmingshanensis sp. nov.
acidothermophilic red algae
genome assembly
heterotrophy
Journal
Journal of phycology
ISSN: 1529-8817
Titre abrégé: J Phycol
Pays: United States
ID NLM: 9882935
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
29
09
2019
accepted:
02
04
2020
entrez:
18
1
2021
pubmed:
19
1
2021
medline:
4
2
2021
Statut:
ppublish
Résumé
The taxonomic placement of strains belonging to the extremophilic red alga Galdieria maxima has been controversial due to the inconsistent phylogenetic position inferred from molecular phylogenetic analyses. Galdieria maxima nom. inval. was classified in this genus based on morphology and molecular data in the early work, but some subsequent molecular phylogenetic analyses have inferred strains of G. maxima to be closely related to the genus Cyanidioschyzon. To address this controversy, an isolated strain identified as G. maxima using the rbcL gene sequence as the genetic barcode was examined using a comprehensive analysis across morphological, physiological, and genomic traits. Herein are reported the chloroplast-, mitochondrion-, and chromosome-level nuclear genome assemblies. Comparative analysis of orthologous gene clusters and genome arrangements suggested that the genome structure of this strain was more similar to that of the generitype of Cyanidioschyzon, C. merolae than to the generitype of Galdieria, G. sulphuraria. While the ability to uptake various forms of organic carbon for growth is an important physiological trait of Galdieria, this strain was identified as an ecologically obligate photoautotroph (i.e., the inability to utilize the natural concentrations of organic carbons) and lacked various gene models predicted as sugar transporters. Based on the genomic, morphological, and physiological traits, we propose this strain to be a new genus and species, Cyanidiococcus yangmingshanensis. Re-evaluation of the 18S rRNA and rbcL gene sequences of the authentic strain of G. maxima, IPPAS-P507, with those of C. yangmingshanensis suggests that the rbcL sequences of "G. maxima" deposited in GenBank correspond to misidentified isolates.
Substances chimiques
RNA, Ribosomal, 16S
0
RNA, Ribosomal, 18S
0
Banques de données
GENBANK
['KY033436.1', 'MN431657', 'MN431656']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1428-1442Subventions
Organisme : Academia Sinica Core Facility and Innovative Instrument Project
ID : AS-CFII-108-114
Pays : International
Organisme : Ministry of Science and Technology
ID : 108-2311-B-110-001
Pays : International
Organisme : Ministry of Science and Technology
ID : 106-2811-B-001-023-MY3
Pays : International
Organisme : Ministry of Science and Technology
ID : 105-2628-B-029-001-MY3
Pays : International
Organisme : National Research Foundation of Korea
ID : NRF-2017R1A2B3001923
Pays : International
Informations de copyright
© 2020 Phycological Society of America.
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