Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages.

Adaptive Immunity Bronchoalveolar Lavage Bronchoalveolar Lavage Fluid CD4-Positive T-Lymphocytes / cytology CD8-Positive T-Lymphocytes / cytology COVID-19 / diagnosis Cell Communication Gene Expression Profiling Humans Immunity, Innate Lung / virology Macrophages, Alveolar / cytology Monocytes / cytology Neutrophils / cytology Phenotype Principal Component Analysis RNA-Seq Single-Cell Analysis Th17 Cells / cytology

Journal

Cell research
ISSN: 1748-7838
Titre abrégé: Cell Res
Pays: England
ID NLM: 9425763

Informations de publication

Date de publication:
03 2021
Historique:
received: 02 07 2020
accepted: 20 11 2020
pubmed: 22 1 2021
medline: 16 3 2021
entrez: 21 1 2021
Statut: ppublish

Résumé

How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated. Here, we perform single-cell deep-immune profiling of bronchoalveolar lavage (BAL) samples from 5 patients with mild and 26 with critical COVID-19 in comparison to BALs from non-COVID-19 pneumonia and normal lung. We use pseudotime inference to build T-cell and monocyte-to-macrophage trajectories and model gene expression changes along them. In mild COVID-19, CD8

Identifiants

DOI: 10.1038/s41422-020-00455-9 PMID: 33473155 PMC: PMC8027624
pubmed: 33473155
doi: 10.1038/s41422-020-00455-9
pii: 10.1038/s41422-020-00455-9
pmc: PMC8027624
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

272-290

Subventions

Organisme : Vlaams Instituut voor Biotechnologie (Flanders Institute for Biotechnology)
ID : VR 2016 2312 Doc.1521/4
Organisme : Stichting Tegen Kanker (Belgian Foundation Against Cancer)
ID : 1833317N
Organisme : Fonds Wetenschappelijk Onderzoek (Research Foundation Flanders)
ID : 1S66020N
Organisme : Fonds Wetenschappelijk Onderzoek (Research Foundation Flanders)
ID : 11E9819N
Organisme : Fonds Wetenschappelijk Onderzoek (Research Foundation Flanders)
ID : 1833317N

Investigateurs

Maria Bosisio (M)
Michael Casaer (M)
Frederik De Smet (F)
Paul De Munter (P)
Stephanie Humblet-Baron (S)
Adrian Liston (A)
Natalie Lorent (N)
Kim Martinod (K)
Paul Proost (P)
Jeroen Raes (J)
Karin Thevissen (K)
Robin Vos (R)
Birgit Weynand (B)
Carine Wouters (C)

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Auteurs

Els Wauters (E)

Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
Department of Pneumology, University Hospitals Leuven, Leuven, Belgium.

Pierre Van Mol (P)

Department of Pneumology, University Hospitals Leuven, Leuven, Belgium.
Laboratory of Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium.
VIB Center for Cancer Biology, VIB, Leuven, Belgium.
ORCID: 0000-0003-1314-9413

Abhishek Dinkarnath Garg (AD)

Laboratory for Cell Stress & Immunity (CSI), Department of Cellular and Molecular Medicine (CMM), KU Leuven, Leuven, Belgium.
ORCID: 0000-0002-9976-9922

Sander Jansen (S)

Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Leuven, Belgium.
ORCID: 0000-0002-9344-131X

Yannick Van Herck (Y)

Laboratory of Experimental Oncology, Department of Oncology, KU Leuven, Leuven, Belgium.

Lore Vanderbeke (L)

Laboratory of Clinical Bacteriology and Mycology, Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium.

Ayse Bassez (A)

Laboratory of Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium.
VIB Center for Cancer Biology, VIB, Leuven, Belgium.

Bram Boeckx (B)

Laboratory of Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium.
VIB Center for Cancer Biology, VIB, Leuven, Belgium.

Bert Malengier-Devlies (B)

Laboratory of Immunobiology, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Leuven, Belgium.
ORCID: 0000-0003-3527-1145

Anna Timmerman (A)

Laboratory of Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium.
VIB Center for Cancer Biology, VIB, Leuven, Belgium.

Thomas Van Brussel (T)

Laboratory of Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium.
VIB Center for Cancer Biology, VIB, Leuven, Belgium.

Tina Van Buyten (T)

Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Leuven, Belgium.

Rogier Schepers (R)

Laboratory of Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium.
VIB Center for Cancer Biology, VIB, Leuven, Belgium.

Elisabeth Heylen (E)

Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Leuven, Belgium.
ORCID: 0000-0003-4359-7225

Dieter Dauwe (D)

Laboratory of Intensive Care Medicine, Department of Cellular and Molecular Medicine, KU Leuven, Leuven, Belgium.
ORCID: 0000-0002-9771-2543

Christophe Dooms (C)

Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
Department of Pneumology, University Hospitals Leuven, Leuven, Belgium.

Jan Gunst (J)

Laboratory of Intensive Care Medicine, Department of Cellular and Molecular Medicine, KU Leuven, Leuven, Belgium.

Greet Hermans (G)

Laboratory of Intensive Care Medicine, Department of Cellular and Molecular Medicine, KU Leuven, Leuven, Belgium.

Philippe Meersseman (P)

Laboratory for Clinical Infectious and Inflammatory Disorders, Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium.

Dries Testelmans (D)

Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
Department of Pneumology, University Hospitals Leuven, Leuven, Belgium.

Jonas Yserbyt (J)

Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
Department of Pneumology, University Hospitals Leuven, Leuven, Belgium.

Sabine Tejpar (S)

Molecular Digestive Oncology, Department of Oncology, KU Leuven, Leuven, Belgium.

Walter De Wever (W)

Department of Imaging & Pathology, KU Leuven, Leuven, Belgium.

Patrick Matthys (P)

Laboratory of Immunobiology, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Leuven, Belgium.
ORCID: 0000-0002-9685-6836

Johan Neyts (J)

Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Leuven, Belgium.

Joost Wauters (J)

Laboratory for Clinical Infectious and Inflammatory Disorders, Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium.

Junbin Qian (J)

Department of Gynecologic Oncology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310006, China. dr_qian@zju.edu.cn.

Diether Lambrechts (D)

Laboratory of Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium. Diether.Lambrechts@kuleuven.vib.be.
VIB Center for Cancer Biology, VIB, Leuven, Belgium. Diether.Lambrechts@kuleuven.vib.be.
ORCID: 0000-0002-3429-302X

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