Biochemical Characterization of Recombinant UDPG-Dependent IAA Glucosyltransferase from Maize (
2,4-Dichlorophenoxyacetic Acid
/ chemistry
Adenosine Triphosphate
/ chemistry
Amino Acid Motifs
Binding Sites
Cations
Enzyme Inhibitors
/ pharmacology
Escherichia coli
/ metabolism
Glucose
/ chemistry
Glucosephosphates
/ chemistry
Glucosyltransferases
/ antagonists & inhibitors
Homeostasis
Hydrogen-Ion Concentration
Kinetics
Molecular Docking Simulation
Plant Growth Regulators
/ metabolism
Recombinant Proteins
/ chemistry
Substrate Specificity
Uridine Diphosphate Glucose
/ chemistry
Zea mays
/ drug effects
UDPG-dependent IAA glucosyltransferase
auxin conjugate
enzyme modulators
indole-3-acetic acid
inhibitor–enzyme interaction
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
25 Mar 2021
25 Mar 2021
Historique:
received:
26
02
2021
revised:
19
03
2021
accepted:
22
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
17
6
2021
Statut:
epublish
Résumé
Here, we report a biochemical characterization of recombinant maize indole-3-acetyl-β-d-glucose (IAGlc) synthase which glucosylates indole-3-acetic acid (IAA) and thus abolishes its auxinic activity affecting plant hormonal homeostasis. Substrate specificity analysis revealed that IAA is a preferred substrate of IAGlc synthase; however, the enzyme can also glucosylate indole-3-butyric acid and indole-3-propionic acid with the relative activity of 66% and 49.7%, respectively.
Identifiants
pubmed: 33805949
pii: ijms22073355
doi: 10.3390/ijms22073355
pmc: PMC8037650
pii:
doi:
Substances chimiques
Cations
0
Enzyme Inhibitors
0
Glucosephosphates
0
Plant Growth Regulators
0
Recombinant Proteins
0
2,4-Dichlorophenoxyacetic Acid
2577AQ9262
Adenosine Triphosphate
8L70Q75FXE
glucose-1-phosphate
CIX3U01VAU
Glucosyltransferases
EC 2.4.1.-
indole-3-acetate beta-glucosyltransferase
EC 2.4.1.121
Glucose
IY9XDZ35W2
Uridine Diphosphate Glucose
V50K1D7P4Y
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Uniwersytet Mikolaja Kopernika w Toruniu
ID : 1190-B
Références
J Exp Bot. 2013 Jun;64(9):2541-55
pubmed: 23580748
Plant Cell. 2010 Aug;22(8):2856-71
pubmed: 20693356
J Chromatogr. 1977 Feb 11;132(2):267-76
pubmed: 188858
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
PLoS One. 2013 Apr 16;8(4):e61705
pubmed: 23613909
Anal Biochem. 1979 Nov 1;99(2):233-41
pubmed: 92899
Phytochemistry. 2009 Feb;70(3):325-47
pubmed: 19217634
Sci Rep. 2021 Jan 13;11(1):1154
pubmed: 33441983
Plant Cell Physiol. 2014 Jan;55(1):218-28
pubmed: 24285754
Microb Cell Fact. 2008 Apr 04;7:11
pubmed: 18394160
Pharmacogenetics. 1997 Aug;7(4):255-69
pubmed: 9295054
Plant Physiol. 1988;88:1474-80
pubmed: 11537438
Genome Biol. 2001;2(2):REVIEWS3004
pubmed: 11182895
J Plant Physiol. 2020 Feb;245:153082
pubmed: 31862648
J Comput Chem. 2009 Dec;30(16):2785-91
pubmed: 19399780
Nat Protoc. 2010 Apr;5(4):725-38
pubmed: 20360767
Biochem Biophys Res Commun. 1980 Mar 28;93(2):588-92
pubmed: 6446303
Rice (N Y). 2019 Dec 18;12(1):92
pubmed: 31853664
Nat Methods. 2015 Jan;12(1):7-8
pubmed: 25549265
BMC Bioinformatics. 2008 Jan 23;9:40
pubmed: 18215316
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W270-7
pubmed: 21624888
Plant J. 2011 Apr;66(1):182-93
pubmed: 21443631
Plant J. 2012 Mar;69(6):1030-42
pubmed: 22077743
Phytochemistry. 2015 Sep;117:25-33
pubmed: 26057226
Curr Opin Struct Biol. 2012 Oct;22(5):540-9
pubmed: 22819665
Biochem J. 1982 Nov 1;207(2):273-81
pubmed: 6218801
Plant Physiol. 1988;88:1481-5
pubmed: 11537439
Plant Physiol. 1990;94:1598-604
pubmed: 11537469
Phytochemistry. 2009 May;70(8):957-69
pubmed: 19524990
Bioinformatics. 2013 Oct 15;29(20):2588-95
pubmed: 23975762
Biochem Biophys Res Commun. 2020 Nov 5;532(2):244-250
pubmed: 32868079
Anal Chem. 1996 Mar 1;68(5):850-8
pubmed: 8779443
J Ind Microbiol Biotechnol. 2013 Apr;40(3-4):257-74
pubmed: 23385853
Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6910-6917
pubmed: 32152121
Plant Cell. 2010 Aug;22(8):2660-79
pubmed: 20798329
Plant Cell Rep. 2019 Jun;38(6):731-739
pubmed: 30903268
J Biol Chem. 2001 Feb 9;276(6):4350-6
pubmed: 11042207
Science. 1994 Sep 16;265(5179):1699-701
pubmed: 8085154
Biochem J. 1991 Oct 15;279 ( Pt 2):509-14
pubmed: 1719957
Plant Mol Biol. 2016 Jan;90(1-2):77-93
pubmed: 26496910
J Exp Bot. 2011 Mar;62(6):1757-73
pubmed: 21307383