Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 05 2021
28 05 2021
Historique:
received:
28
08
2020
accepted:
27
04
2021
entrez:
29
5
2021
pubmed:
30
5
2021
medline:
9
6
2021
Statut:
epublish
Résumé
Recent studies have demonstrated that drought leads to dramatic, highly conserved shifts in the root microbiome. At present, the molecular mechanisms underlying these responses remain largely uncharacterized. Here we employ genome-resolved metagenomics and comparative genomics to demonstrate that carbohydrate and secondary metabolite transport functionalities are overrepresented within drought-enriched taxa. These data also reveal that bacterial iron transport and metabolism functionality is highly correlated with drought enrichment. Using time-series root RNA-Seq data, we demonstrate that iron homeostasis within the root is impacted by drought stress, and that loss of a plant phytosiderophore iron transporter impacts microbial community composition, leading to significant increases in the drought-enriched lineage, Actinobacteria. Finally, we show that exogenous application of iron disrupts the drought-induced enrichment of Actinobacteria, as well as their improvement in host phenotype during drought stress. Collectively, our findings implicate iron metabolism in the root microbiome's response to drought and may inform efforts to improve plant drought tolerance to increase food security.
Identifiants
pubmed: 34050180
doi: 10.1038/s41467-021-23553-7
pii: 10.1038/s41467-021-23553-7
pmc: PMC8163885
doi:
Substances chimiques
Iron
E1UOL152H7
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3209Subventions
Organisme : NIEHS NIH HHS
ID : P42 ES007373
Pays : United States
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