Evaluation of the eazyplex® EHEC complete (Amplex) assay for the differentiation of stx1 and stx2 positivity in stool samples.

Bacteriological Techniques / methods Enterohemorrhagic Escherichia coli / isolation & purification Escherichia coli Infections / diagnosis Feces / microbiology Molecular Diagnostic Techniques / methods Shiga Toxin Shiga Toxin 1 / genetics Shiga Toxin 2 / genetics Shiga-Toxigenic Escherichia coli
Enterohemorrhagic Escherichia coli Loop-mediated amplification Shiga toxin Shiga toxin-producing Escherichia coli

Journal

Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883

Informations de publication

Date de publication:
09 2021
Historique:
received: 19 05 2021
revised: 15 07 2021
accepted: 15 07 2021
pubmed: 27 7 2021
medline: 29 12 2021
entrez: 26 7 2021
Statut: ppublish

Résumé

The performance of the eazyplex® EHEC complete (Amplex) for the detection of Shiga toxin genes in stool samples was evaluated. The assay performed well in distinguishing between stx1 and stx2 but suboptimal sensitivity may limit its use to complementary testing rather than primary diagnosis of Shiga toxin-producing Escherichia coli infections.

Identifiants

DOI: 10.1016/j.mimet.2021.106291 PMID: 34311019
pubmed: 34311019
pii: S0167-7012(21)00159-7
doi: 10.1016/j.mimet.2021.106291
pii:
doi:

Substances chimiques

Shiga Toxin 1 0
Shiga Toxin 2 0
Shiga Toxin 75757-64-1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

106291

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Hannes Bjarki Vigfússon (HB)

Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, 701 85 Örebro, Sweden. Electronic address: hannes.vigfusson@regionorebrolan.se.

Theresa Ennefors (T)

Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, 701 85 Örebro, Sweden.

Torbjörn Kjerstadius (T)

Department of Clinical Microbiology, Central Hospital, 651 85 Karlstad, Sweden.

Martin Sundqvist (M)

Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, 701 85 Örebro, Sweden.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques microbiologiques › Techniques bactériologiques › Evaluation of the eazyplex® EHEC complete...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli producteur de Shiga-toxine › Escherichia coli entérohémorrhagique › Evaluation of the eazyplex® EHEC complete...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Enterobacteriaceae › Infections à Escherichia coli › Evaluation of the eazyplex® EHEC complete...
questionsmedicales.fr › Liquides et sécrétions biologiques › Fèces › Evaluation of the eazyplex® EHEC complete...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de diagnostic moléculaire › Evaluation of the eazyplex® EHEC complete...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Entérotoxines › Shiga-toxines › Shiga-toxine › Evaluation of the eazyplex® EHEC complete...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Entérotoxines › Shiga-toxines › Shiga-toxine-1 › Evaluation of the eazyplex® EHEC complete...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Entérotoxines › Shiga-toxines › Shiga-toxine-2 › Evaluation of the eazyplex® EHEC complete...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli producteur de Shiga-toxine › Evaluation of the eazyplex® EHEC complete...