De novo assembly, annotation, and comparative analysis of 26 diverse maize genomes.
Centromere
/ genetics
Chromosome Mapping
Chromosomes, Plant
DNA Methylation
Disease Resistance
/ genetics
Genes, Plant
Genetic Variation
Genome, Plant
Genotype
High-Throughput Nucleotide Sequencing
Molecular Sequence Annotation
Multifactorial Inheritance
/ genetics
Phenotype
Plant Diseases
Polymorphism, Single Nucleotide
Regulatory Sequences, Nucleic Acid
Sequence Analysis, DNA
Tetraploidy
Transcriptome
Whole Genome Sequencing
Zea mays
/ genetics
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
06 08 2021
06 08 2021
Historique:
received:
14
01
2021
accepted:
24
06
2021
entrez:
6
8
2021
pubmed:
7
8
2021
medline:
21
8
2021
Statut:
ppublish
Résumé
We report de novo genome assemblies, transcriptomes, annotations, and methylomes for the 26 inbreds that serve as the founders for the maize nested association mapping population. The number of pan-genes in these diverse genomes exceeds 103,000, with approximately a third found across all genotypes. The results demonstrate that the ancient tetraploid character of maize continues to degrade by fractionation to the present day. Excellent contiguity over repeat arrays and complete annotation of centromeres revealed additional variation in major cytological landmarks. We show that combining structural variation with single-nucleotide polymorphisms can improve the power of quantitative mapping studies. We also document variation at the level of DNA methylation and demonstrate that unmethylated regions are enriched for cis-regulatory elements that contribute to phenotypic variation.
Identifiants
pubmed: 34353948
pii: 373/6555/655
doi: 10.1126/science.abg5289
pmc: PMC8733867
mid: NIHMS1753799
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
655-662Subventions
Organisme : NIH HHS
ID : S10 OD028632
Pays : United States
Informations de copyright
Copyright © 2021, American Association for the Advancement of Science.
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