Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).
Australia
Citrus
/ anatomy & histology
Citrus sinensis
/ anatomy & histology
Diploidy
Fruit
/ genetics
Gene Expression Regulation, Plant
Hybrid Cells
/ cytology
Microsatellite Repeats
/ genetics
Plant Breeding
/ methods
Plant Leaves
/ anatomy & histology
Polymorphism, Genetic
Protoplasts
/ cytology
Tetraploidy
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
26
03
2021
accepted:
23
07
2021
entrez:
10
8
2021
pubmed:
11
8
2021
medline:
15
12
2021
Statut:
epublish
Résumé
The Australian finger lime is a unique citrus species that has gained importance due to its unique fruit characteristics and perceived tolerance to Huanglongbing (HLB), an often-fatal disease of citrus trees. In this study, we developed allotetraploid finger lime hybrids and cybrids by utilizing somatic cell fusion techniques to fuse diploid 'OLL8' sweet orange or 'Page' tangelo callus-derived protoplasts with finger lime (FL) mesophyll-derived protoplasts. Six somatic fusions were regenerated from the 'OLL8' + FL fusion, while three putative cybrids were regenerated from the 'Page' + FL fusion. Ploidy levels and nuclear-expressed sequence tag derived simple sequence repeat (EST-SSR) markers confirmed the somatic hybrid production, and mitochondrial DNA primer sets confirmed the cybrid nature. Several trees produced by the somatic fusion remained HLB negative even after 6 years of growth in an HLB-endemic environment. Pathogenesis related (PR) and other genes that are often upregulated in HLB-tolerant trees were also upregulated in our somatic fusions. These newly developed somatic fusions and cybrids could potentially be used as breeding parents to develop the next generation of improved HLB-tolerant rootstocks and scions.
Identifiants
pubmed: 34375348
doi: 10.1371/journal.pone.0255842
pii: PONE-D-21-10046
pmc: PMC8354479
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0255842Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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