Overexpression of arogenate dehydratase reveals an upstream point of metabolic control in phenylalanine biosynthesis.
Carbohydrate Epimerases
/ genetics
Flowers
/ genetics
Gene Expression Regulation, Plant
Hydro-Lyases
/ genetics
Mutation
Petunia
/ genetics
Phenylalanine
/ biosynthesis
Plant Proteins
/ genetics
Plants, Genetically Modified
Plastids
/ genetics
Secondary Metabolism
/ genetics
Shikimic Acid
/ metabolism
Petunia hybrida
arogenate dehydratase
d-ribulose-5-phosphate 3-epimerase
pentose phosphate pathway
phenylalanine
phenylalanine biosynthesis
shikimate pathway
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
20
05
2021
accepted:
12
08
2021
pubmed:
18
8
2021
medline:
27
1
2022
entrez:
17
8
2021
Statut:
ppublish
Résumé
Out of the three aromatic amino acids, the highest flux in plants is directed towards phenylalanine, which is utilized to synthesize proteins and thousands of phenolic metabolites contributing to plant fitness. Phenylalanine is produced predominantly in plastids via the shikimate pathway and subsequent arogenate pathway, both of which are subject to complex transcriptional and post-transcriptional regulation. Previously, it was shown that allosteric feedback inhibition of arogenate dehydratase (ADT), which catalyzes the final step of the arogenate pathway, restricts flux through phenylalanine biosynthesis. Here, we show that in petunia (Petunia hybrida) flowers, which typically produce high phenylalanine levels, ADT regulation is relaxed, but not eliminated. Moderate expression of a feedback-insensitive ADT increased flux towards phenylalanine, while high overexpression paradoxically reduced phenylalanine formation. This reduction could be partially, but not fully, recovered by bypassing other known metabolic flux control points in the aromatic amino acid network. Using comparative transcriptomics, reverse genetics, and metabolic flux analysis, we discovered that transcriptional regulation of the d-ribulose-5-phosphate 3-epimerase gene in the pentose phosphate pathway controls flux into the shikimate pathway. Taken together, our findings reveal that regulation within and upstream of the shikimate pathway shares control over phenylalanine biosynthesis in the plant cell.
Substances chimiques
Plant Proteins
0
Shikimic Acid
29MS2WI2NU
Phenylalanine
47E5O17Y3R
Hydro-Lyases
EC 4.2.1.-
pretyrosine dehydratase
EC 4.2.1.-
Carbohydrate Epimerases
EC 5.1.3.-
ribulosephosphate 3-epimerase
EC 5.1.3.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
737-751Informations de copyright
© 2021 Society for Experimental Biology and John Wiley & Sons Ltd.
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