Identification and molecular characterization of a novel carlavirus infecting rose plants (Rosa chinensis Jacq.).
Journal
Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
05
05
2021
accepted:
23
08
2021
pubmed:
13
10
2021
medline:
18
1
2022
entrez:
12
10
2021
Statut:
ppublish
Résumé
In the present study, the genome sequence of a potential novel virus, tentatively named "rose virus C" (RVC), was mined from publically available transcriptomic data from a Rosa chinensis plant. The complete genome sequence of RVC consists of 8,386 nt, excluding a 3' poly(A) tail, and contains five ORFs. Phylogenetic analysis showed that RVC clustered with members of the genus Carlavirus, family Betaflexiviridae. The replicase gene had 48.8-52.1% nt sequence identity to those of other carlaviruses, while the CP gene had 40.4-45.9% nt sequence identity, which is far below the species demarcation cutoff of 72%. The incidence of RVC in rose plants was low (5.4%). Overall, our data suggest that RVC is a novel atypical virus of the genus Carlavirus.
Identifiants
pubmed: 34636953
doi: 10.1007/s00705-021-05260-2
pii: 10.1007/s00705-021-05260-2
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3499-3502Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
Références
Adams MJ, Antoniw JF, Bar-Joseph M et al (2004) Virology division news: the new plant virus family flexiviridae and assessment of molecular criteria for species demarcation. Arch Virol 149:1045–1060
doi: 10.1007/s00705-004-0304-0
Adams MJ, Candresse T, Hammond J et al (2011) Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier, Oxford, pp 920–941
Diaz-Lara A, Mollov D, Golino D et al (2020) Complete genome sequence of rose virus A, the first carlavirus identified in rose. Arch Virol 165:241–244
doi: 10.1007/s00705-019-04460-1
He Y, Yang ZK, Hong N et al (2015) Deep sequencing reveals a novel closterovirus associated with wild rose leaf rosette disease. Mol Plant Pathol 16:449–458
doi: 10.1111/mpp.12202
Martelli GP, Adams MJ, Kreuze JF et al (2007) Family Flexiviridae: a case study in virion and genome plasticity. Ann Rev Phytopathol 45:153–157
doi: 10.1146/annurev.phyto.45.062806.094401
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
pubmed: 3447015
pmcid: 3447015
Tamura K, Stecher G, Peterson D et al (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
doi: 10.1093/molbev/mst197
Wang Z, Gerstein M, Snyder M et al (2009) RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet 10:57–63
doi: 10.1038/nrg2484
Xing F, Gao DH, Liu H et al (2020) Molecular characterization and pathogenicity test of prunus necrotic ringspot virus isolates from China rose (Rosa chinensis Jacq.). Arch Virol 165:2479–2486
doi: 10.1007/s00705-020-04739-8
Xing F, Gao DH, Wang HQ et al (2021) Molecular characterization of rose spring dwarf-associated virus isolated from China rose (Rosa chinensis Jacq.). Arch Virol 166:2059–2062
doi: 10.1007/s00705-021-05064-4
Zhang XQ, Liu SY, Yang YZ et al (2020) Virus identification and detection by high-throughput sequencing and RT-PCR from rose plants in Beijing. Acta Phytopathol Sin. https://doi.org/10.13926/j.cnki.apps.000481
doi: 10.13926/j.cnki.apps.000481