Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host.
biochemistry
chemical biology
electron transfer protein
escherichia coli
horizontal gene transfer
infectious disease
iron-sulfur enzyme
microbial engineering
microbiology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
04 03 2022
04 03 2022
Historique:
received:
02
06
2021
accepted:
03
02
2022
entrez:
4
3
2022
pubmed:
5
3
2022
medline:
30
4
2022
Statut:
epublish
Résumé
Iron-sulfur (Fe-S) clusters are ancient and ubiquitous protein cofactors and play irreplaceable roles in many metabolic and regulatory processes. Fe-S clusters are built and distributed to Fe-S enzymes by dedicated protein networks. The core components of these networks are widely conserved and highly versatile. However, Fe-S proteins and enzymes are often inactive outside their native host species. We sought to systematically investigate the compatibility of Fe-S networks with non-native Fe-S enzymes. By using collections of Fe-S enzyme orthologs representative of the entire range of prokaryotic diversity, we uncovered a striking correlation between phylogenetic distance and probability of functional expression. Moreover, coexpression of a heterologous Fe-S biogenesis pathway increases the phylogenetic range of orthologs that can be supported by the foreign host. We also find that Fe-S enzymes that require specific electron carrier proteins are rarely functionally expressed unless their taxon-specific reducing partners are identified and co-expressed. We demonstrate how these principles can be applied to improve the activity of a radical
Identifiants
pubmed: 35244541
doi: 10.7554/eLife.70936
pii: 70936
pmc: PMC8896826
doi:
pii:
Substances chimiques
Escherichia coli Proteins
0
Iron-Sulfur Proteins
0
Sulfur
70FD1KFU70
Iron
E1UOL152H7
Banques de données
figshare
['10.6084/m9.figshare.13664927.v1']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022, D'Angelo et al.
Déclaration de conflit d'intérêts
FD, EF, PG, HS, MP, RM, FB, SL, Nv, ND, Cd, MP, EB, SG, BP, SO, FB, GB No competing interests declared
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