Genome-wide identification and analysis of the GUB_WAK_bind gene family in Gossypium hirsutum.
Bioinformatics analysis
Cotton
GUB_WAK_bind
Gene family
Salt stress
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
received:
03
02
2022
accepted:
04
04
2022
pubmed:
21
4
2022
medline:
14
7
2022
entrez:
20
4
2022
Statut:
ppublish
Résumé
Upland cotton is one of the main cultivated species of cotton, and salt stress is an important factor in its growth and development. Wall-associated receptor kinase galacturonan binding (GUB_WAK_bind) is an extracellular domain of wall-associated kinase (WAK), which can sense the environment and play a role in the response to plant stress. In this study, the GUB_WAK_bind gene in Gossypium hirsutum was identified and analyzed by bioinformatics at the whole genome level, including its physicochemical properties, evolutionary development, gene structure, chromosome positioning, cis-acting elements in the promoter, etc., and the expression of the GUB_WAK_bind genes under salt stress were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). A total of 22 GUB_WAK_bind gene members were identified in Gossypium hirsutum and divided into three subgroups by evolutionary development and motif analysis, most of which contained motif 5, which is similar to the motif pattern of subgroup members. The number of exons in this gene family is between 1 and 4, the number of introns is between 0 and 3, and 22 gene members are distributed on 14 chromosomes of Gossypium hirsutum. Almost all gene members have adverse stress response elements in their promoter region. The expression analysis in response to salt stress showed that the selected six genes were induced by NaCl stress with significant expression differences (P < 0.05). The results of this study have a certain reference value for understanding the evolution and function of GUB_WAK_bind genes and studying the salt tolerance genes of Gossypium hirsutum.
Sections du résumé
BACKGROUND
BACKGROUND
Upland cotton is one of the main cultivated species of cotton, and salt stress is an important factor in its growth and development. Wall-associated receptor kinase galacturonan binding (GUB_WAK_bind) is an extracellular domain of wall-associated kinase (WAK), which can sense the environment and play a role in the response to plant stress.
METHODS AND RESULTS
RESULTS
In this study, the GUB_WAK_bind gene in Gossypium hirsutum was identified and analyzed by bioinformatics at the whole genome level, including its physicochemical properties, evolutionary development, gene structure, chromosome positioning, cis-acting elements in the promoter, etc., and the expression of the GUB_WAK_bind genes under salt stress were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). A total of 22 GUB_WAK_bind gene members were identified in Gossypium hirsutum and divided into three subgroups by evolutionary development and motif analysis, most of which contained motif 5, which is similar to the motif pattern of subgroup members. The number of exons in this gene family is between 1 and 4, the number of introns is between 0 and 3, and 22 gene members are distributed on 14 chromosomes of Gossypium hirsutum. Almost all gene members have adverse stress response elements in their promoter region. The expression analysis in response to salt stress showed that the selected six genes were induced by NaCl stress with significant expression differences (P < 0.05).
CONCLUSIONS
CONCLUSIONS
The results of this study have a certain reference value for understanding the evolution and function of GUB_WAK_bind genes and studying the salt tolerance genes of Gossypium hirsutum.
Identifiants
pubmed: 35441355
doi: 10.1007/s11033-022-07449-3
pii: 10.1007/s11033-022-07449-3
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6405-6413Subventions
Organisme : Key Technologies Research and Development Program
ID : 2021YFE0101200
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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