Functional divergence in the proteins encoded by ARO80 from S. uvarum, S. kudriavzevii and S. cerevisiae explain differences in the aroma production during wine fermentation.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
19
04
2022
received:
23
01
2022
accepted:
24
04
2022
pubmed:
11
5
2022
medline:
30
7
2022
entrez:
10
5
2022
Statut:
ppublish
Résumé
Phenylethanol (PE) and phenylethyl acetate (PEA) are commonly desired compounds in wine because of their rose-like aroma. The yeast S. cerevisiae produces the PE either through de novo biosynthesis by shikimate pathway followed by the Ehrlich pathway or the direct phenylalanine catabolism via Ehrlich pathway, and then converted into PEA. Previous work demonstrated that, compared to S. cerevisiae, other Saccharomyces species, such as S. kudriavzevii and S. uvarum, produce higher concentrations of PE and PEA from the precursor phenylalanine, which indicates differential activities of the biosynthetic-involved enzymes. A previous in-silico analysis suggested that the transcriptional activator Aro80p is one of the best candidates to explain these differences. An improved functional analysis identified significant radical amino acid changes in the S. uvarum and S. kudriavzevii Aro80p that could impact the expression of the catabolic genes ARO9 and ARO10, and hence, the production of PE from phenylalanine. Indeed, wine S. cerevisiae strains carrying the S. uvarum and S. kudriavzevii ARO80 alleles increased the production of both compounds in the presence of phenylalanine by increasing the expression of ARO9 and ARO10. This study provides novel insights of the unidentified Aro80p regulatory region and the potential usage of alternatives ARO80 alleles to enhance the PE and PEA concentration in wine.
Identifiants
pubmed: 35536034
doi: 10.1111/1751-7915.14071
pmc: PMC9328738
doi:
Substances chimiques
Acetates
0
Phenylalanine
47E5O17Y3R
Phenylethyl Alcohol
ML9LGA7468
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2281-2291Informations de copyright
© 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
Références
Biotechnol Bioeng. 2014 Jan;111(1):115-24
pubmed: 23836015
Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20024-9
pubmed: 22123960
J Appl Microbiol. 2013 May;114(5):1405-14
pubmed: 23294204
Nucleic Acids Res. 1991 Nov 11;19(21):5991-7
pubmed: 1945883
Microb Cell Fact. 2016 Mar 12;15:51
pubmed: 26971319
Nucleic Acids Res. 1993 Jul 11;21(14):3329-30
pubmed: 8341614
Nat Protoc. 2007;2(1):1-4
pubmed: 17401330
FEMS Yeast Res. 2017 Aug 1;17(5):
pubmed: 28505256
Front Genet. 2019 Feb 12;10:82
pubmed: 30809248
Trends Biochem Sci. 2005 Jul;30(7):405-12
pubmed: 15950477
Mol Cell Biol. 2007 Apr;27(8):3065-86
pubmed: 17308034
PLoS Comput Biol. 2009 Apr;5(4):e1000344
pubmed: 19343224
Mol Cell Biol. 1999 May;19(5):3360-71
pubmed: 10207060
Environ Microbiol. 2019 Nov;21(11):4076-4091
pubmed: 31336027
EMBO J. 2003 Oct 1;22(19):5147-53
pubmed: 14517252
Nucleic Acids Res. 1996 Dec 1;24(23):4599-607
pubmed: 8967907
PLoS Genet. 2021 Nov 11;17(11):e1009872
pubmed: 34762651
Cell. 1993 Feb 26;72(4):575-85
pubmed: 8440021
Microbiol Mol Biol Rev. 2003 Mar;67(1):1-15, table of contents
pubmed: 12626680
J Microbiol Methods. 2016 Aug;127:203-205
pubmed: 27327211
Front Microbiol. 2016 Jun 07;7:897
pubmed: 27375606
Front Genet. 2019 Mar 13;10:187
pubmed: 30930934
Appl Microbiol Biotechnol. 2012 Nov;96(3):601-18
pubmed: 22940803
Nat Biotechnol. 2014 Dec;32(12):1262-7
pubmed: 25184501
G3 (Bethesda). 2011 Jun;1(1):11-25
pubmed: 22384314
Nucleic Acids Res. 1996 Jul 1;24(13):2519-24
pubmed: 8692690
Yeast. 2000 Jun 15;16(8):675-729
pubmed: 10861899
Appl Environ Microbiol. 2008 Apr;74(8):2259-66
pubmed: 18281432
Front Microbiol. 2016 Mar 31;7:435
pubmed: 27064588
Crit Rev Biotechnol. 2019 Mar;39(2):235-248
pubmed: 30570367
Science. 1974 Sep 6;185(4154):862-4
pubmed: 4843792
Genetics. 1996 Apr;142(4):1069-82
pubmed: 8846888
Nature. 2009 Mar 19;458(7236):337-41
pubmed: 19212322
Science. 1996 Oct 25;274(5287):546, 563-7
pubmed: 8849441
Mol Microbiol. 2013 Jun;88(6):1120-34
pubmed: 23651256
Microbiol Mol Biol Rev. 2006 Sep;70(3):583-604
pubmed: 16959962
J Biol Chem. 1997 Aug 1;272(31):19383-92
pubmed: 9235937
Adv Food Nutr Res. 2018;85:177-210
pubmed: 29860974
Int J Food Microbiol. 2007 May 1;116(1):11-8
pubmed: 17346840
Int J Food Microbiol. 2015 Jul 2;204:101-10
pubmed: 25867085
Int J Food Microbiol. 2015 Jul 16;205:41-6
pubmed: 25886016