Genome characterization and complete sequence of a new badnavirus from Pandanus amaryllifolius.
Journal
Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
received:
01
03
2022
accepted:
10
04
2022
pubmed:
25
5
2022
medline:
29
6
2022
entrez:
24
5
2022
Statut:
ppublish
Résumé
A new badnavirus was sequenced from fragrant pandan grass (Pandanus amaryllifolius) displaying mosaic and chlorosis on the leaves. The complete genome sequence was determined by high-throughput sequencing. The new badnavirus was tentatively named "pandanus mosaic associated virus" (PMaV). Similar to those of other members of the genus Badnavirus, the genome of PMaV consists of a circular DNA molecule of 7,481 bp with three open reading frames (ORF) potentially coding for three proteins. ORF3 encodes a polyprotein with conserved protein domains including zinc finger, trimeric dUTPase, aspartic protease, reverse transcriptase (RT), and RNase H domains. Pairwise comparisons of the highly conserved RT + RNase H region revealed the highest nucleotide (nt) sequence identity (70.71%) to taro bacilliform CH virus-Et17 (MG017324). In addition to PMaV, viral sequences corresponding to orchid fleck dichorhavirus (OFV) were detected in the same plant sample. The complete sequence of the OFV coding region shared >98% nt sequence identity with other isolates of OFV available in the GenBank database. Disease symptoms could not be attributed exclusively to PMaV or OFV, as both viruses were present in the pandan grass exhibiting mosaic and chlorosis.
Identifiants
pubmed: 35610515
doi: 10.1007/s00705-022-05480-0
pii: 10.1007/s00705-022-05480-0
doi:
Substances chimiques
Ribonuclease H
EC 3.1.26.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1717-1720Informations de copyright
© 2022. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.
Références
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