Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq.
Journal
Nature biotechnology
ISSN: 1546-1696
Titre abrégé: Nat Biotechnol
Pays: United States
ID NLM: 9604648
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
14
07
2021
accepted:
08
04
2022
pubmed:
1
6
2022
medline:
12
10
2022
entrez:
31
5
2022
Statut:
ppublish
Résumé
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
Identifiants
pubmed: 35637419
doi: 10.1038/s41587-022-01312-3
pii: 10.1038/s41587-022-01312-3
pmc: PMC9546769
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1447-1451Informations de copyright
© 2022. The Author(s).
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