A direct enzymatic evaluation platform (DEEP) to fine-tuning pyridoxal 5'-phosphate-dependent proteins for cadaverine production.
CRISPRi
cadaverine
cofactor engineering
high-throughput screening
lysine decarboxylase
pyridoxal 5′-phosphate (PLP)
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
revised:
06
10
2022
received:
25
08
2022
accepted:
17
10
2022
pubmed:
23
10
2022
medline:
9
2
2023
entrez:
22
10
2022
Statut:
ppublish
Résumé
Pyridoxal 5'-phosphate (pyridoxal phosphate, PLP) is an essential cofactor for multiple enzymatic reactions in industry. However, cofactor engineering based on PLP regeneration and related to the performance of enzymes in chemical production has rarely been discussed. First, we found that MG1655 strain was sensitive to nitrogen source and relied on different amino acids, thus the biomass was significantly reduced when PLP excess in the medium. Then, the six KEIO collection strains were applied to find out the prominent gene in deoxyxylulose-5-phosphate (DXP) pathway, where pdxB was superior in controlling cell growth. Therefore, the clustered regularly interspaced short palindromic repeats interference (CRISPRi) targeted on pdxB in MG1655 was employed to establish a novel direct enzymatic evaluation platform (DEEP) as a high-throughput tool and obtained the optimal modules for incorporating of PLP to enhance the biomass and activity of PLP-dependent enzymes simultaneously. As a result, the biomass has increased by 55% using P
Substances chimiques
Cadaverine
L90BEN6OLL
Pyridoxal Phosphate
5V5IOJ8338
Phosphates
0
pdxB protein, E coli
EC 1.1.1.-
Carbohydrate Dehydrogenases
EC 1.1.-
Escherichia coli Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
272-283Subventions
Organisme : Ministry of Science and Technology, Taiwan
Informations de copyright
© 2022 Wiley Periodicals LLC.
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