Characterization of the novel temperate Staphylococcus haemolyticus phage IME1365_01.
Journal
Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870
Informations de publication
Date de publication:
07 Jan 2023
07 Jan 2023
Historique:
received:
03
07
2022
accepted:
03
10
2022
entrez:
7
1
2023
pubmed:
8
1
2023
medline:
11
1
2023
Statut:
epublish
Résumé
The presence of a novel functional prophage, IME1365_01, was predicted from bacterial high-throughput sequencing data and then successfully induced from Staphylococcus haemolyticus by mitomycin C treatment. Transmission electron microscopy showed that phage IME1365_01 has an icosahedral head (43 nm in diameter) and a long tail (172 nm long). This phage possesses a double-stranded DNA genome of 44,875 bp with a G+C content of 35.35%. A total of 63 putative open reading frames (ORFs) were identified in its genome. BLASTn analysis revealed that IME1365_01 is similar to Staphylococcus phage vB_SepS_E72, but with a genome homology coverage of only 26%. The phage genome does not have fixed termini. In ORF24 of phage IME1365_01, a conserved Toll-interleukin-1 receptor domain of the TIR_2 superfamily (accession no. c123749) is located at its N-terminus, and this might serve as a component of an anti-bacterial system. In conclusion, we developed a platform to obtain active temperate phage from prediction, identification, and induction from its bacterial host. After mass screening using this platform, numerous temperate phages and their innate anti-bacterial elements can provide extensive opportunities for therapy against bacterial (especially drug-resistant bacterial) infections.
Identifiants
pubmed: 36609576
doi: 10.1007/s00705-022-05650-0
pii: 10.1007/s00705-022-05650-0
doi:
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
41Subventions
Organisme : Key Project of Beijing University of Chemical Technology
ID : No. XK1803-06
Organisme : National Key Research and Development Program of China
ID : No. 2020YFC2005405
Organisme : Funds for First-class Discipline Construction
ID : No. XK1805
Organisme : Fundamental Research Funds for Central Universities of the Central South University
ID : No. BUCTRC201917
Organisme : Fundamental Research Funds for Central Universities of the Central South University
ID : BUCTZY2022
Organisme : Military Biosecurity Research Program
ID : No. 20SWAQX27
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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