Large-scale analysis of putative Euphorbiaceae R2R3-MYB transcription factors identifies a MYB involved in seed oil biosynthesis.
Euphorbiaceae
Evolutionary rate
Gene feature
MYB
Ortholog
Paralog
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
17 Mar 2023
17 Mar 2023
Historique:
received:
30
10
2022
accepted:
10
03
2023
entrez:
17
3
2023
pubmed:
18
3
2023
medline:
21
3
2023
Statut:
epublish
Résumé
MYB transcription factors are widely distributed in the plant kingdom and play key roles in regulatory networks governing plant metabolism and biochemical and physiological processes. Here, we first determined the R2R3-MYB genes in five Euphorbiaceae genomes. The three Trp (W) residues from the first MYB domain (R2) were absolutely conserved, whereas the first W residue from the second MYB domain (R3) was preferentially mutated. The R2R3-MYBs were clustered into 48 functional subfamilies, of which 34 had both R2R3-MYBs of Euphorbiaceae species and AtMYBs, and four contained only Euphorbiaceae R2R3-MYBs. The whole-genome duplication (WGD) and/or segmental duplication (SD) played key roles in the expansion of the R2R3-MYB family. Unlike paralogous R2R3-MYB family members, orthologous R2R3-MYB members contained a higher selective pressure and were subject to a constrained evolutionary rate. VfMYB36 was specifically expressed in fruit, and its trend was consistent with the change in oil content, indicating that it might be involved in oil biosynthesis. Overexpression experiments showed that VfMYB36 could significantly provide linolenic acid (C18:3) content, which eventually led to a significant increase in oil content. Our study first provides insight into understanding the evolution and expression of R2R3-MYBs in Euphorbiaceae species, and also provides a target for the production of biomass diesel and a convenient way for breeding germplasm resources with high linolenic acid content in the future.
Sections du résumé
BACKGROUND
BACKGROUND
MYB transcription factors are widely distributed in the plant kingdom and play key roles in regulatory networks governing plant metabolism and biochemical and physiological processes.
RESULTS
RESULTS
Here, we first determined the R2R3-MYB genes in five Euphorbiaceae genomes. The three Trp (W) residues from the first MYB domain (R2) were absolutely conserved, whereas the first W residue from the second MYB domain (R3) was preferentially mutated. The R2R3-MYBs were clustered into 48 functional subfamilies, of which 34 had both R2R3-MYBs of Euphorbiaceae species and AtMYBs, and four contained only Euphorbiaceae R2R3-MYBs. The whole-genome duplication (WGD) and/or segmental duplication (SD) played key roles in the expansion of the R2R3-MYB family. Unlike paralogous R2R3-MYB family members, orthologous R2R3-MYB members contained a higher selective pressure and were subject to a constrained evolutionary rate. VfMYB36 was specifically expressed in fruit, and its trend was consistent with the change in oil content, indicating that it might be involved in oil biosynthesis. Overexpression experiments showed that VfMYB36 could significantly provide linolenic acid (C18:3) content, which eventually led to a significant increase in oil content.
CONCLUSION
CONCLUSIONS
Our study first provides insight into understanding the evolution and expression of R2R3-MYBs in Euphorbiaceae species, and also provides a target for the production of biomass diesel and a convenient way for breeding germplasm resources with high linolenic acid content in the future.
Identifiants
pubmed: 36927311
doi: 10.1186/s12870-023-04163-5
pii: 10.1186/s12870-023-04163-5
pmc: PMC10022305
doi:
Substances chimiques
Transcription Factors
0
alpha-Linolenic Acid
0RBV727H71
Plant Proteins
0
Plant Oils
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
145Subventions
Organisme : National Natural Science Foundation of China
ID : 32201602
Organisme : Natural Science Fund Youth Project of Hunan Province
ID : 2021JJ41067
Organisme : Outstanding Youth of the Education Department of Hunan Province
ID : 20B624
Informations de copyright
© 2023. The Author(s).
Références
BMC Plant Biol. 2022 Aug 24;22(1):411
pubmed: 36002818
Phytochemistry. 2021 May;185:112686
pubmed: 33582587
Int J Mol Sci. 2022 Mar 22;23(7):
pubmed: 35408785
Trends Plant Sci. 2007 Oct;12(10):444-51
pubmed: 17855156
Nat Protoc. 2016 Sep;11(9):1650-67
pubmed: 27560171
Curr Opin Plant Biol. 2001 Oct;4(5):447-56
pubmed: 11597504
BMC Plant Biol. 2012 Jul 09;12:106
pubmed: 22776508
BMC Plant Biol. 2022 Dec 6;22(1):569
pubmed: 36471247
J Plant Res. 2005 Feb;118(1):61-9
pubmed: 15703854
Plant J. 2022 Sep;111(5):1340-1353
pubmed: 35785503
Plant J. 2023 Feb;113(3):521-535
pubmed: 36534067
Nucleic Acids Res. 2020 Jan 10;48(1):460-471
pubmed: 31733060
Int J Biol Macromol. 2022 Nov 30;221:796-805
pubmed: 36037910
Genomics Proteomics Bioinformatics. 2010 Mar;8(1):77-80
pubmed: 20451164
Nucleic Acids Res. 2012 Apr;40(7):e49
pubmed: 22217600
Trends Genet. 1997 Feb;13(2):67-73
pubmed: 9055608
Plant Cell. 1993 Dec;5(12):1795-805
pubmed: 8305872
Front Plant Sci. 2020 Oct 16;11:571881
pubmed: 33178243
Bioinformatics. 2014 May 1;30(9):1236-40
pubmed: 24451626
Nucleic Acids Res. 2013 Jan;41(Database issue):D1152-8
pubmed: 23180799
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Nucleic Acids Res. 2013 Jul;41(12):e121
pubmed: 23598997
Plant J. 2011 Apr;66(1):94-116
pubmed: 21443626
Plant Cell Physiol. 2010 Jun;51(6):1084-90
pubmed: 20427511
Plant Cell Environ. 2020 Nov;43(11):2712-2726
pubmed: 32799321
Cell. 1982 Dec;31(2 Pt 1):453-63
pubmed: 6297766
Biomolecules. 2019 Sep 16;9(9):
pubmed: 31527450
Genomics Proteomics Bioinformatics. 2019 Dec;17(6):558-575
pubmed: 32224189
J Genet. 2015 Feb 20;94(1):e5-9
pubmed: 25810403
J Exp Bot. 2012 Apr;63(7):2541-56
pubmed: 22301384
Nat Commun. 2014 Jun 30;5:4106
pubmed: 24978855
Mol Plant. 2015 May;8(5):689-708
pubmed: 25840349
Nucleic Acids Res. 2018 Jan 4;46(D1):D493-D496
pubmed: 29040681
Plant Cell Physiol. 2015 Dec;56(12):2436-46
pubmed: 26508520
Biology (Basel). 2020 Mar 24;9(3):
pubmed: 32213912
Nucleic Acids Res. 2019 Jul 2;47(W1):W256-W259
pubmed: 30931475
BMC Plant Biol. 2022 Dec 9;22(1):572
pubmed: 36482301
Nat Biotechnol. 2015 Mar;33(3):290-5
pubmed: 25690850
Nat Protoc. 2006;1(2):641-6
pubmed: 17406292
Gene. 2004 Feb 4;326:13-22
pubmed: 14729259
Hortic Res. 2021 Apr 1;8(1):77
pubmed: 33790254
Proc Natl Acad Sci U S A. 2012 Jul 24;109(30):12219-24
pubmed: 22778405
Front Genet. 2020 Dec 03;11:550677
pubmed: 33343619
Physiol Plant. 2021 Jun;172(2):847-868
pubmed: 33180329
Biochemistry. 1999 Feb 9;38(6):1921-9
pubmed: 10026273
Trends Plant Sci. 2013 May;18(5):267-76
pubmed: 23040085
Plant Physiol. 2017 Feb;173(2):1211-1225
pubmed: 27932421
Int J Biol Macromol. 2022 May 31;208:509-519
pubmed: 35341887
Plant Physiol. 2020 Jun;183(2):637-655
pubmed: 32291329
Mol Biol Evol. 2015 Jan;32(1):268-74
pubmed: 25371430
Front Plant Sci. 2016 Apr 29;7:577
pubmed: 27200050
Nucleic Acids Res. 2021 Jan 8;49(D1):D412-D419
pubmed: 33125078
Physiol Plant. 2021 Nov;173(3):1163-1178
pubmed: 34363225
BMC Plant Biol. 2022 Jun 8;22(1):281
pubmed: 35676625
Cell. 2017 Jan 26;168(3):442-459.e20
pubmed: 28111071
J Mol Evol. 1998 Jan;46(1):74-83
pubmed: 9419227
BMC Plant Biol. 2022 Oct 8;22(1):479
pubmed: 36209052
Physiol Mol Biol Plants. 2021 Jun;27(6):1323-1335
pubmed: 34177150
Int J Mol Sci. 2021 Jun 07;22(11):
pubmed: 34200125
PLoS One. 2012;7(6):e37463
pubmed: 22719841
Plant Cell Physiol. 2016 Apr;57(4):824-47
pubmed: 26872835
Plant Cell Physiol. 1995 Dec;36(8):1405-20
pubmed: 8589926
Nat Methods. 2017 Jun;14(6):587-589
pubmed: 28481363
Eur J Biochem. 1999 Jun;262(2):247-57
pubmed: 10336605
BMC Plant Biol. 2022 Nov 14;22(1):529
pubmed: 36376794
BMC Plant Biol. 2008 Jul 22;8:83
pubmed: 18647406
BMC Genomics. 2012 Oct 10;13:544
pubmed: 23050870
Nucleic Acids Res. 2005 Jan 20;33(2):511-8
pubmed: 15661851
BMC Plant Biol. 2022 Nov 28;22(1):547
pubmed: 36443672
Plant Mol Biol. 2018 Sep;98(1-2):1-18
pubmed: 30167900