Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network.
Legionella non pneumophila
PFGE
RAPD-PCR
Rep-PCR
SAU-PCR
mip-gene sequencing
Journal
Environmental monitoring and assessment
ISSN: 1573-2959
Titre abrégé: Environ Monit Assess
Pays: Netherlands
ID NLM: 8508350
Informations de publication
Date de publication:
22 Mar 2023
22 Mar 2023
Historique:
received:
14
10
2022
accepted:
02
03
2023
entrez:
22
3
2023
pubmed:
23
3
2023
medline:
25
3
2023
Statut:
epublish
Résumé
Understanding the actual distribution of different Legionella species in water networks would help prevent outbreaks. Culture investigations followed by serological agglutination tests, with poly/monovalent antisera, still represent the gold standard for isolation and identification of Legionella strains. However, also MALDI-TOF and mip-gene sequencing are currently used. This study was conducted to genetically correlate strains of Legionella non pneumophila (L-np) isolated during environmental surveillance comparing different molecular techniques. Overall, 346 water samples were collected from the water system of four pavilions located in a hospital of the Apulia Region of Italy. Strains isolated from the samples were then identified by serological tests, MALDI-TOF, and mip-gene sequencing. Overall, 24.9% of water samples were positive for Legionella, among which the majority were Legionella pneumophila (Lpn) 1 (52.3%), followed by Lpn2-15 (20.9%), L-np (17.4%), Lpn1 + Lpn2-15 (7.1%), and L-np + Lpn1 (2.3%). Initially, L-np strains were identified as L. bozemanii by monovalent antiserum, while MALDI-TOF and mip-gene sequencing assigned them to L. anisa. More cold water than hot water samples were contaminated by L. anisa (p < 0.001). PFGE, RAPD, Rep-PCR, and SAU-PCR were performed to correlate L. anisa strains. Eleven out of 14 strains identified in all four pavilions showed 100% of similarity upon PFGE analysis. RAPD, Rep-PCR, and SAU-PCR showed greater discriminative power than PFGE.
Identifiants
pubmed: 36947259
doi: 10.1007/s10661-023-11078-z
pii: 10.1007/s10661-023-11078-z
pmc: PMC10033568
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
496Informations de copyright
© 2023. The Author(s).
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