Identification of MYB gene family and functional analysis of GhMYB4 in cotton (Gossypium spp.).
Cotton
Expression profiles
Gossypium spp
MYB genes
Pigment gland development
Journal
Molecular genetics and genomics : MGG
ISSN: 1617-4623
Titre abrégé: Mol Genet Genomics
Pays: Germany
ID NLM: 101093320
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
received:
01
06
2021
accepted:
05
03
2023
medline:
28
4
2023
pubmed:
8
4
2023
entrez:
7
4
2023
Statut:
ppublish
Résumé
Myeloblastosis (MYB) transcription factors (TFs) form a large gene family involved in a variety of biological processes in plants. Little is known about their roles in the development of cotton pigment glands. In this study, 646 MYB members were identified in Gossypium hirsutum genome and phylogenetic classification was analyzed. Evolution analysis revealed assymetric evolution of GhMYBs during polyploidization and sequence divergence of MYBs in G. hirustum was preferentially happend in D sub-genome. WGCNA (weighted gene co-expression network analysis) showed that four modules had potential relationship with gland development or gossypol biosynthesis in cotton. Eight differentially expressed GhMYB genes were identified by screening transcriptome data of three pairs of glanded and glandless cotton lines. Of these, four were selected as candidate genes for cotton pigment gland formation or gossypol biosynthesis by qRT-PCR assay. Silencing of GH_A11G1361 (GhMYB4) downregulated expression of multiple genes in gossypol biosynthesis pathway, indicating it could be involved in gossypol biosynthesis. The potential protein interaction network suggests that several MYBs may have indirect interaction with GhMYC2-like, a key regulator of pigment gland formation. Our study was the systematic analysis of MYB genes in cotton pigment gland development, providing candidate genes for further study on the roles of cotton MYB genes in pigment gland formation, gossypol biosynthesis and future crop plant improvement.
Identifiants
pubmed: 37027022
doi: 10.1007/s00438-023-02005-5
pii: 10.1007/s00438-023-02005-5
doi:
Substances chimiques
Gossypol
KAV15B369O
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
755-766Subventions
Organisme : National Key R and D Plan of China
ID : No. 2018YFD0100402
Organisme : National Natural Science Foundation of China
ID : No. 31621005
Organisme : National Natural Science Foundation of China
ID : No. 31901581
Organisme : Central Public-interest Scientific Institution Basic Research Fund
ID : No.1610162021013
Organisme : Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences, and the United States Department of Agriculture-Agricultural Research Service
ID : USDA-ARS Project No. 3091-210000-44-00D
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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