Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum.
Amine production
Co-cultivation
Corynebacterium glutamicum
Formamidase
Formamide
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
received:
11
04
2023
accepted:
12
05
2023
revised:
09
05
2023
medline:
3
7
2023
pubmed:
29
5
2023
entrez:
29
5
2023
Statut:
ppublish
Résumé
Formamide is rarely used as nitrogen source by microorganisms. Therefore, formamide and formamidase have been used as protection system to allow for growth under non-sterile conditions and for non-sterile production of acetoin, a product lacking nitrogen. Here, we equipped Corynebacterium glutamicum, a renowned workhorse for industrial amino acid production for 60 years, with formamidase from Helicobacter pylori 26695, enabling growth with formamide as sole nitrogen source. Thereupon, the formamide/formamidase system was exploited for efficient formamide-based production of the nitrogenous compounds L-glutamate, L-lysine, N-methylphenylalanine, and dipicolinic acid by transfer of the formamide/formamidase system to established producer strains. Stable isotope labeling verified the incorporation of nitrogen from formamide into biomass and the representative product L-lysine. Moreover, we showed ammonium leakage during formamidase-based access of formamide to be exploitable to support growth of formamidase-deficient C. glutamicum in co-cultivation and demonstrated that efficient utilization of formamide as sole nitrogen source benefitted from overexpression of formate dehydrogenase. KEY POINTS: • C. glutamicum was engineered to access formamide. • Formamide-based production of nitrogenous compounds was established. • Nitrogen cross-feeding supported growth of a formamidase-negative strain.
Identifiants
pubmed: 37246985
doi: 10.1007/s00253-023-12592-3
pii: 10.1007/s00253-023-12592-3
pmc: PMC10313556
doi:
Substances chimiques
Lysine
K3Z4F929H6
Amines
0
Amino Acids
0
Nitrogen
N762921K75
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4245-4260Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : ForceYield (031B0825C)
Organisme : Bundesministerium für Bildung und Forschung
ID : BMBF project ForceYield (031B0825B)
Informations de copyright
© 2023. The Author(s).
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