A special satellite-like RNA of a novel hypovirus from Pestalotiopsis fici broadens the definition of fungal satellite.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
06 2023
06 2023
Historique:
received:
21
09
2022
accepted:
23
05
2023
revised:
20
06
2023
medline:
22
6
2023
pubmed:
7
6
2023
entrez:
7
6
2023
Statut:
epublish
Résumé
Satellites associated with plant or animal viruses have been largely detected and characterized, while those from mycoviruses together with their roles remain far less determined. Three dsRNA segments (dsRNA 1 to 3 termed according to their decreasing sizes) were identified in a strain of phytopathogenic fungus Pestalotiopsis fici AH1-1 isolated from a tea leaf. The complete sequences of dsRNAs 1 to 3, with the sizes of 10316, 5511, and 631 bp, were determined by random cloning together with a RACE protocol. Sequence analyses support that dsRNA1 is a genome of a novel hypovirus belonging to genus Alphahypovirus of the family Hypoviridae, tentatively named Pestalotiopsis fici hypovirus 1 (PfHV1); dsRNA2 is a defective RNA (D-RNA) generating from dsRNA1 with septal deletions; and dsRNA3 is the satellite component of PfHV1 since it could be co-precipitated with other dsRNA components in the same sucrose fraction by ultra-centrifuge, suggesting that it is encapsulated together with PfHV1 genomic dsRNAs. Moreover, dsRNA3 shares an identical stretch (170 bp) with dsRNAs 1 and 2 at their 5' termini and the remaining are heterogenous, which is distinct from a typical satellite that generally has very little or no sequence similarity with helper viruses. More importantly, dsRNA3 lacks a substantial open reading frame (ORF) and a poly (A) tail, which is unlike the known satellite RNAs of hypoviruses, as well as unlike those in association with Totiviridae and Partitiviridae since the latters are encapsidated in coat proteins. As up-regulated expression of RNA3, dsRNA1 was significantly down-regulated, suggesting that dsRNA3 negatively regulates the expression of dsRNA1, whereas dsRNAs 1 to 3 have no obvious impact on the biological traits of the host fungus including morphologies and virulence. This study indicates that PfHV1 dsRNA3 is a special type of satellite-like nucleic acid that has substantial sequence homology with the host viral genome without encapsidation in a coat protein, which broadens the definition of fungal satellite.
Identifiants
pubmed: 37285391
doi: 10.1371/journal.ppat.1010889
pii: PPATHOGENS-D-22-01644
pmc: PMC10281576
doi:
Substances chimiques
RNA, Satellite
0
RNA, Double-Stranded
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010889Informations de copyright
Copyright: © 2023 Han et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Viruses. 2018 May 13;10(5):
pubmed: 29757259
Adv Virus Res. 2004;63:423-72
pubmed: 15530566
Nucleic Acids Res. 2019 Sep 5;47(15):8255-8271
pubmed: 31269212
Plant Dis. 2017 Oct;101(10):1802-1811
pubmed: 30676920
Virus Res. 2012 May;165(2):143-50
pubmed: 22366520
Virology. 2014 Sep;464-465:441-449
pubmed: 25108682
Virology. 2016 Jun;493:75-85
pubmed: 27015523
Curr Opin Virol. 2018 Dec;33:96-105
pubmed: 30144641
Virology. 1995 Apr 1;208(1):58-66
pubmed: 11831731
Environ Microbiol. 2018 Apr;20(4):1464-1483
pubmed: 29411500
Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10647-51
pubmed: 1961731
Res Virol. 1995 Jan-Feb;146(1):61-7
pubmed: 7538689
Adv Virus Res. 2013;86:109-47
pubmed: 23498905
Virology. 2000 Oct 10;276(1):181-9
pubmed: 11022006
Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4911-8
pubmed: 26283371
J Virol. 1992 May;66(5):3069-76
pubmed: 1560537
Virology. 2014 Sep;464-465:450-459
pubmed: 25104554
Nature. 2004 Sep 30;431(7008):582-6
pubmed: 15457264
Virology. 2015 Jul;481:151-60
pubmed: 25781585
Nat Commun. 2017 Aug 1;8(1):168
pubmed: 28761042
Arch Virol. 2014 Jul;159(7):1861-3
pubmed: 24473711
Virology. 1993 Jun;194(2):576-84
pubmed: 8503176
Stud Mycol. 2014 Sep;79:121-86
pubmed: 25492988
Microbiol Rev. 1996 Mar;60(1):250-65
pubmed: 8852903
Virology. 2001 Mar 1;281(1):117-23
pubmed: 11222102
Acta Biochim Biophys Sin (Shanghai). 2007 Mar;39(3):217-23
pubmed: 17342261
EMBO J. 1991 Apr;10(4):741-6
pubmed: 2009855
J Virol. 1995 Nov;69(11):6892-7
pubmed: 7474105
Front Microbiol. 2019 May 15;10:1076
pubmed: 31156589
Nat Rev Microbiol. 2005 Aug;3(8):632-42
pubmed: 16064055
Virology. 1995 Oct 1;212(2):574-86
pubmed: 7571427
J Gen Virol. 2018 May;99(5):615-616
pubmed: 29589826
J Gen Virol. 1990 May;71 ( Pt 5):1153-62
pubmed: 2161049
Front Microbiol. 2020 Feb 19;11:240
pubmed: 32140150
Virus Res. 2013 Jun;174(1-2):69-77
pubmed: 23499998
J Virol. 2006 Mar;80(5):2566-74
pubmed: 16474162
Nucleic Acids Res. 2003 Dec 15;31(24):7117-30
pubmed: 14654687
Phytopathology. 2004 Sep;94(9):917-23
pubmed: 18943067
Virus Res. 2016 Feb 2;213:332-342
pubmed: 26547008
Mol Biol Evol. 2021 Jun 25;38(7):3022-3027
pubmed: 33892491
mBio. 2020 May 26;11(3):
pubmed: 32457242
ISME J. 2021 Jul;15(7):1893-1906
pubmed: 33531623
J Virol. 2015 May;89(9):5060-71
pubmed: 25694604
Nature. 2016 Dec 22;540(7634):539-543
pubmed: 27880757
Virology. 1991 Aug;183(2):747-52
pubmed: 1853573
Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16749-54
pubmed: 18922782
J Biol Chem. 1991 Oct 15;266(29):19419-25
pubmed: 1918054
J Virol. 2009 Feb;83(4):1981-91
pubmed: 19073734
Virus Res. 2013 Nov 6;177(2):171-8
pubmed: 23973915
mBio. 2018 Mar 20;9(2):
pubmed: 29559577
Virology. 2011 Sep 15;418(1):49-56
pubmed: 21813149
Virology. 1991 Aug;183(2):479-86
pubmed: 1830181