High-throughput and reliable assessments of the ionization constant of monoprotic organic acids through an arginine based mixed mode HPLC.
Acidity
Arginine stationary phase
Dissociation constant
High throughput analysis
PKa assessment
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
25 Oct 2023
25 Oct 2023
Historique:
received:
08
06
2023
revised:
19
07
2023
accepted:
24
07
2023
medline:
11
9
2023
pubmed:
3
8
2023
entrez:
2
8
2023
Statut:
ppublish
Résumé
The charge state of a molecule is the single most prominent attribute ruling out its interactions with the surrounding environment. In a previous study, the retention of acids on the new Celeris™ Arginine (ARG) column was found to be predominantly driven by electrostatics and, specifically, their charge state. Therefore, we analysed 41 compounds in liquid chromatography with ultraviolet detection to study possible relationships between the analytical retention on this phase and the pK
Identifiants
pubmed: 37531733
pii: S0731-7085(23)00373-4
doi: 10.1016/j.jpba.2023.115604
pii:
doi:
Substances chimiques
Arginine
94ZLA3W45F
Organic Chemicals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115604Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Scott Anderson was the Scientific Director of Regis Technologies Inc., that developed and manufactured the Celeris™ Arginine column, whose application is described in the present study.