Activation of secondary metabolite gene clusters in Chaetomium olivaceum via the deletion of a histone deacetylase.
Chaetomium olivaceum
Biosynthetic gene clusters
Epigenetic regulation
HDAC
Polyketide synthase
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
11 May 2024
11 May 2024
Historique:
received:
09
01
2024
accepted:
04
05
2024
revised:
23
04
2024
medline:
12
5
2024
pubmed:
12
5
2024
entrez:
11
5
2024
Statut:
epublish
Résumé
Histone acetylation modifications in filamentous fungi play a crucial role in epigenetic gene regulation and are closely linked to the transcription of secondary metabolite (SM) biosynthetic gene clusters (BGCs). Histone deacetylases (HDACs) play a pivotal role in determining the extent of histone acetylation modifications and act as triggers for the expression activity of target BGCs. The genus Chaetomium is widely recognized as a rich source of novel and bioactive SMs. Deletion of a class I HDAC gene of Chaetomium olivaceum SD-80A, g7489, induces a substantial pleiotropic effect on the expression of SM BGCs. The C. olivaceum SD-80A ∆g7489 strain exhibited significant changes in morphology, sporulation ability, and secondary metabolic profile, resulting in the emergence of new compound peaks. Notably, three polyketides (A1-A3) and one asterriquinone (A4) were isolated from this mutant strain. Furthermore, our study explored the BGCs of A1-A4, confirming the function of two polyketide synthases (PKSs). Collectively, our findings highlight the promising potential of molecular epigenetic approaches for the elucidation of novel active compounds and their biosynthetic elements in Chaetomium species. This finding holds great significance for the exploration and utilization of Chaetomium resources. KEY POINTS: • Deletion of a class I histone deacetylase activated secondary metabolite gene clusters. • Three polyketides and one asterriquinone were isolated from HDAC deleted strain. • Two different PKSs were reported in C. olivaceum SD-80A.
Identifiants
pubmed: 38734756
doi: 10.1007/s00253-024-13173-8
pii: 10.1007/s00253-024-13173-8
doi:
Substances chimiques
Histone Deacetylases
EC 3.5.1.98
Polyketides
0
Polyketide Synthases
79956-01-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
332Subventions
Organisme : Key R&D Program of Shandong Province, China
ID : 2022SFGC0105
Organisme : Natural Science Foundation of Shandong Province
ID : ZR2022QC186
Organisme : Natural Science Foundation of Shandong Province
ID : ZR2021QB173
Organisme : Natural Science Foundation of Shandong Province
ID : ZR2021LSW022
Organisme : Young Taishan Scholarship to Xuekui Xia
ID : tsqn202103100
Organisme : Jinan Talent Project for Universities
ID : 2021GXRC062
Organisme : Jinan Talent Project for Universities
ID : 202228088
Organisme : Key innovation Project and Science, Education and Industry Integration Innovation Pilot Project Qilu University of Technology (Shandong Academy of Sciences)
ID : 2022PX051
Organisme : Education and Industry Integration Innovation Pilot Project Qilu University of Technology (Shandong Academy of Sciences)
ID : 2022PX027
Organisme : Education and Industry Integration Innovation Pilot Project Qilu University of Technology (Shandong Academy of Sciences)
ID : 2022JBZ01-06
Organisme : Research Project for Talents Qilu University of Technology (Shandong Academy of Sciences)
ID : 2023RCKY14
Informations de copyright
© 2024. The Author(s).
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