Analysis of chitinase gene family in barley and function study of HvChi22 involved in drought tolerance.
Hordeum
/ genetics
Chitinases
/ genetics
Droughts
Gene Expression Regulation, Plant
/ genetics
Stress, Physiological
/ genetics
Phylogeny
Multigene Family
Plant Proteins
/ genetics
Arabidopsis
/ genetics
Promoter Regions, Genetic
/ genetics
Plants, Genetically Modified
/ genetics
Gene Expression Profiling
/ methods
Drought Resistance
Hordeum vulgare
Chitinase gene family
Gene overexpression
Reactive oxygen species
Water deficiency
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
13 Jun 2024
13 Jun 2024
Historique:
received:
02
04
2024
accepted:
17
05
2024
medline:
13
6
2024
pubmed:
13
6
2024
entrez:
13
6
2024
Statut:
epublish
Résumé
Chitinase (Chi) is a pathogenesis-related protein, also reported to play an important role in plant responses to abiotic stress. However, its role in response to abiotic stress in barley is still unclear. In this study, a total of 61 Chi gene family members were identified from the whole genome of wild barley EC_S1. Phylogenetic analysis suggested that these family genes were divided into five groups. Among these genes, four pairs of collinearity genes were discovered. Besides, abundant cis-regulatory elements, including drought response element and abscisic acid response element were identified in the promoter regions of HvChi gene family members. The expression profiles revealed that most HvChi family members were significantly up-regulated under drought stress, which was also validated by RT-qPCR measurements. To further explore the role of Chi under drought stress, HvChi22 was overexpressed in Arabidopsis. Compared to wild-type plants, overexpression of HvChi22 enhanced drought tolerance by increasing the activity of oxidative protective enzymes, which caused less MDA accumulation. Our study improved the understanding of the Chi gene family under drought stress in barley, and provided a theoretical basis for crop improvement strategies to address the challenges posed by changing environmental conditions.
Sections du résumé
BACKGROUND
BACKGROUND
Chitinase (Chi) is a pathogenesis-related protein, also reported to play an important role in plant responses to abiotic stress. However, its role in response to abiotic stress in barley is still unclear.
RESULTS
RESULTS
In this study, a total of 61 Chi gene family members were identified from the whole genome of wild barley EC_S1. Phylogenetic analysis suggested that these family genes were divided into five groups. Among these genes, four pairs of collinearity genes were discovered. Besides, abundant cis-regulatory elements, including drought response element and abscisic acid response element were identified in the promoter regions of HvChi gene family members. The expression profiles revealed that most HvChi family members were significantly up-regulated under drought stress, which was also validated by RT-qPCR measurements. To further explore the role of Chi under drought stress, HvChi22 was overexpressed in Arabidopsis. Compared to wild-type plants, overexpression of HvChi22 enhanced drought tolerance by increasing the activity of oxidative protective enzymes, which caused less MDA accumulation.
CONCLUSION
CONCLUSIONS
Our study improved the understanding of the Chi gene family under drought stress in barley, and provided a theoretical basis for crop improvement strategies to address the challenges posed by changing environmental conditions.
Identifiants
pubmed: 38869677
doi: 10.1007/s11033-024-09651-x
pii: 10.1007/s11033-024-09651-x
doi:
Substances chimiques
Chitinases
EC 3.2.1.14
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
731Subventions
Organisme : National Natural Science Foundation of China
ID : 32372052
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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