Genome-wide identification and expression analysis of GRAS gene family in Eucalyptus grandis.
Abiotic stress
Eucalyptus grandis
GRAS family
Genome-wide analysis
Phytohormone
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
18 Jun 2024
18 Jun 2024
Historique:
received:
30
10
2023
accepted:
11
06
2024
medline:
19
6
2024
pubmed:
19
6
2024
entrez:
18
6
2024
Statut:
epublish
Résumé
The GRAS gene family is a class of plant-specific transcription factors with important roles in many biological processes, such as signal transduction, disease resistance and stress tolerance, plant growth and development. So far, no information available describes the functions of the GRAS genes in Eucalyptus grandis. A total of 82 GRAS genes were identified with amino acid lengths ranging from 267 to 817 aa, and most EgrGRAS genes had one exon. Members of the GRAS gene family of Eucalyptus grandis are divided into 9 subfamilies with different protein structures, while members of the same subfamily have similar gene structures and conserved motifs. Moreover, these EgrGRAS genes expanded primarily due to segmental duplication. In addition, cis-acting element analysis showed that this family of genes was involved involved in the signal transduction of various plant hormones, growth and development, and stress response. The qRT-PCR data indicated that 18 EgrGRAS genes significantly responded to hormonal and abiotic stresses. Among them, the expression of EgrGRAS13, EgrGRAS68 and EgrGRAS55 genes was significantly up-regulated during the treatment period, and it was hypothesised that members of the EgrGRAS family play an important role in stress tolerance. In this study, the phylogenetic relationship, conserved domains, cis-elements and expression patterns of GRAS gene family of Eucalyptus grandis were analyzed, which filled the gap in the identification of GRAS gene family of Eucalyptus grandis and laid the foundation for analyzing the function of EgrGRAS gene in hormone and stress response.
Sections du résumé
BACKGROUND
BACKGROUND
The GRAS gene family is a class of plant-specific transcription factors with important roles in many biological processes, such as signal transduction, disease resistance and stress tolerance, plant growth and development. So far, no information available describes the functions of the GRAS genes in Eucalyptus grandis.
RESULTS
RESULTS
A total of 82 GRAS genes were identified with amino acid lengths ranging from 267 to 817 aa, and most EgrGRAS genes had one exon. Members of the GRAS gene family of Eucalyptus grandis are divided into 9 subfamilies with different protein structures, while members of the same subfamily have similar gene structures and conserved motifs. Moreover, these EgrGRAS genes expanded primarily due to segmental duplication. In addition, cis-acting element analysis showed that this family of genes was involved involved in the signal transduction of various plant hormones, growth and development, and stress response. The qRT-PCR data indicated that 18 EgrGRAS genes significantly responded to hormonal and abiotic stresses. Among them, the expression of EgrGRAS13, EgrGRAS68 and EgrGRAS55 genes was significantly up-regulated during the treatment period, and it was hypothesised that members of the EgrGRAS family play an important role in stress tolerance.
CONCLUSIONS
CONCLUSIONS
In this study, the phylogenetic relationship, conserved domains, cis-elements and expression patterns of GRAS gene family of Eucalyptus grandis were analyzed, which filled the gap in the identification of GRAS gene family of Eucalyptus grandis and laid the foundation for analyzing the function of EgrGRAS gene in hormone and stress response.
Identifiants
pubmed: 38890621
doi: 10.1186/s12870-024-05288-x
pii: 10.1186/s12870-024-05288-x
doi:
Substances chimiques
Plant Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
573Subventions
Organisme : the National Key Research and Development Program of China during the 14th five-year plan Period
ID : 2022YFD2200203
Organisme : the National Key Research and Development Program of China during the 14th five-year plan Period
ID : 2022YFD2200203
Organisme : the National Key Research and Development Program of China during the 14th five-year plan Period
ID : 2022YFD2200203
Organisme : the National Key Research and Development Program of China during the 14th five-year plan Period
ID : 2022YFD2200203
Organisme : the National Key Research and Development Program of China during the 14th five-year plan Period
ID : 2022YFD2200203
Organisme : the Provincial Natural Resources Fund
ID : 2208085QC92
Organisme : the Project of Introducing and Stabilizing Talents of Anhui agricultural university
ID : rc372109
Organisme : the Innovation and entrepreneurship training program for university students
ID : X202310364233
Informations de copyright
© 2024. The Author(s).
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