Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
Jun 2024
Jun 2024
Historique:
received:
02
08
2023
accepted:
25
04
2024
medline:
20
6
2024
pubmed:
20
6
2024
entrez:
20
6
2024
Statut:
ppublish
Résumé
Actinomycetes are a phylogenetically diverse bacterial group which are widely distributed across terrestrial and aquatic ecosystems. Within this order, the genus Pseudonocardia and their specialised metabolites have been the focus of previous ecological studies due to their antagonistic interactions with other microorganisms and their mutualistic interactions with insects. However, the chemical ecology of free-living Pseudonocardia remains understudied. This study applies a multi-omics approach to investigate the chemical ecology of free-living actinomycetes from the genus Pseudonocardia. In a comparative genomics analysis, it was observed that the biosynthetic gene cluster family distribution was influenced mainly by phylogenetic distance rather than the geographic or ecological origin of strains. This finding was also observed in the mass spectrometry-based metabolomic profiles of nine Pseudonocardia species isolated from marine sediments and two terrestrial species. Antagonist interactions between these 11 species were examined, and matrix-assisted laser desorption/ionisation-mass spectrometry imaging was used to examine in situ chemical interactions between the Southern Ocean strains and their phylogenetically close relatives. Overall, it was demonstrated that phylogeny was the main predictor of antagonistic interactions among free-living Pseudonocardia. Moreover, two features at m/z 441.15 and m/z 332.20 were identified as metabolites related to these interspecies interactions.
Identifiants
pubmed: 38899724
doi: 10.1111/1462-2920.16635
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e16635Subventions
Organisme : Ministerio de Ciencia Tecnología y Telecomunicaciones, Costa Rica
ID : 2-1-4-17-1-037
Organisme : Novo Nordisk Fonden
ID : NNF19OC0055625
Organisme : Danmarks Grundforskningsfond
ID : DNRF137
Informations de copyright
© 2024 The Author(s). Environmental Microbiology published by John Wiley & Sons Ltd.
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