Discovery of Vibrio cholerae in Urban Sewage in Copenhagen, Denmark.
Vibrio cholerae
Metagenomic genome recovery
Pathogen detection
Sewage surveillance
Journal
Microbial ecology
ISSN: 1432-184X
Titre abrégé: Microb Ecol
Pays: United States
ID NLM: 7500663
Informations de publication
Date de publication:
31 Jul 2024
31 Jul 2024
Historique:
received:
13
06
2024
accepted:
23
07
2024
medline:
1
8
2024
pubmed:
1
8
2024
entrez:
31
7
2024
Statut:
epublish
Résumé
We report the discovery of a persistent presence of Vibrio cholerae at very low abundance in the inlet of a single wastewater treatment plant in Copenhagen, Denmark at least since 2015. Remarkably, no environmental or locally transmitted clinical case of V. cholerae has been reported in Denmark for more than 100 years. We, however, have recovered a near-complete genome out of 115 metagenomic sewage samples taken over the past 8 years, despite the extremely low relative abundance of one V. cholerae read out of 500,000 sequenced reads. Due to the very low relative abundance, routine screening of the individual samples did not reveal V. cholerae. The recovered genome lacks the gene responsible for cholerae toxin production, but although this strain may not pose an immediate public health risk, our finding illustrates the importance, challenges, and effectiveness of wastewater-based pathogen surveillance.
Identifiants
pubmed: 39085652
doi: 10.1007/s00248-024-02419-7
pii: 10.1007/s00248-024-02419-7
doi:
Substances chimiques
Sewage
0
Wastewater
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102Subventions
Organisme : Novo Nordisk Fonden
ID : NNF16OC0021856
Organisme : Novo Nordisk Fonden
ID : NNF16OC0021856
Organisme : Novo Nordisk Fonden
ID : NNF16OC0021856
Organisme : Novo Nordisk Fonden
ID : NNF16OC0021856
Organisme : Horizon 2020 Framework Programme
ID : VEO 874735
Organisme : Horizon 2020 Framework Programme
ID : VEO 874735
Organisme : Horizon 2020 Framework Programme
ID : VEO 874735
Organisme : Horizon 2020 Framework Programme
ID : VEO 874735
Organisme : Horizon 2020 Framework Programme
ID : VEO 874735
Organisme : Horizon 2020 Framework Programme
ID : VEO 874735
Informations de copyright
© 2024. The Author(s).
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