The role of FRIGIDA and FLOWERING LOCUS C genes in flowering time of Brassica rapa leafy vegetables.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
25 09 2019
Historique:
received: 16 05 2019
accepted: 03 09 2019
entrez: 27 9 2019
pubmed: 27 9 2019
medline: 30 10 2020
Statut: epublish

Résumé

There is a wide variation of flowering time among lines of Brassica rapa L. Most B. rapa leafy (Chinese cabbage etc.) or root (turnip) vegetables require prolonged cold exposure for flowering, known as vernalization. Premature bolting caused by low temperature leads to a reduction in the yield/quality of these B. rapa vegetables. Therefore, high bolting resistance is an important breeding trait, and understanding the molecular mechanism of vernalization is necessary to achieve this goal. In this study, we demonstrated that BrFRIb functions as an activator of BrFLC in B. rapa. We showed a positive correlation between the steady state expression levels of the sum of the BrFLC paralogs and the days to flowering after four weeks of cold treatment, suggesting that this is an indicator of the vernalization requirement. We indicate that BrFLCs are repressed by the accumulation of H3K27me3 and that the spreading of H3K27me3 promotes stable FLC repression. However, there was no clear relationship between the level of H3K27me3 in the BrFLC and the vernalization requirement. We also showed that if there was a high vernalization requirement, the rate of repression of BrFLC1 expression following prolonged cold treatments was lower.

Identifiants

pubmed: 31554847
doi: 10.1038/s41598-019-50122-2
pii: 10.1038/s41598-019-50122-2
pmc: PMC6761103
doi:

Substances chimiques

Histones 0
MADS Domain Proteins 0
Plant Proteins 0
histone H3 trimethyl Lys4 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

13843

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Auteurs

Satoko Takada (S)

Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe, 657-8501, Japan.

Ayasha Akter (A)

Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe, 657-8501, Japan.

Etsuko Itabashi (E)

Institute of Vegetable and Floriculture Science, NARO, Kusawa, Ano, Tsu, Mie, 514-2392, Japan.

Namiko Nishida (N)

Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe, 657-8501, Japan.

Daniel J Shea (DJ)

Graduate School of Science and Technology, Niigata University, Ikarashi-ninocho, Niigata, 950-2181, Japan.

Naomi Miyaji (N)

Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe, 657-8501, Japan.

Hasan Mehraj (H)

Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe, 657-8501, Japan.

Kenji Osabe (K)

Plant Epigenetics Unit, Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa, 904-0495, Japan.

Motoki Shimizu (M)

Iwate Biotechnology Research Center, Narita, Kitakami, Iwate, 024-0003, Japan.

Takeshi Takasaki-Yasuda (T)

Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe, 657-8501, Japan.

Tomohiro Kakizaki (T)

Institute of Vegetable and Floriculture Science, NARO, Kusawa, Ano, Tsu, Mie, 514-2392, Japan.

Keiichi Okazaki (K)

Graduate School of Science and Technology, Niigata University, Ikarashi-ninocho, Niigata, 950-2181, Japan.

Elizabeth S Dennis (ES)

CSIRO Agriculture and Food, Canberra, ACT 2601, Australia.
University of Technology, Sydney, PO Box 123, Broadway, NSW, 2007, Australia.

Ryo Fujimoto (R)

Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe, 657-8501, Japan. leo@people.kobe-u.ac.jp.

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Classifications MeSH