NanoString nCounter Technology: High-Throughput RNA Validation.
Chimeric RNA
DNA
Gene expression
Molecular barcode
NanoString
Probe hybridization
RNA
nCounter
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
16
11
2019
pubmed:
16
11
2019
medline:
31
12
2020
Statut:
ppublish
Résumé
NanoString nCounter is a multiplex nucleic acid hybridization technology that enables reliable and reproducible assessment of the expression of up to 800 genes or 228 gene fusions in 12 samples in a single assay. The technique works well with a variety of starting materials from fresh or formalin-fixed tissues, cell lysates or biological fluid samples. As low as 1 ng RNA input per sample is needed. In addition to gene expression, copy number variation, single nucleotide variation and chimeric RNAs can be analyzed. In this chapter, we discuss the design, setup, and performance of a NanoString-based RNA assay for gene expression or fusion transcript assessment.
Identifiants
pubmed: 31728967
doi: 10.1007/978-1-4939-9904-0_10
doi:
Substances chimiques
Molecular Probes
0
RNA
63231-63-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM