Characterization of antifungal metabolites produced by Lactobacillus plantarum and Lactobacillus coryniformis isolated from rice rinsed water.

Acetates / isolation & purification Antifungal Agents / isolation & purification Aspergillus / drug effects Chromatography, Reverse-Phase DNA, Fungal / genetics DNA, Ribosomal / genetics Food Preservation Gas Chromatography-Mass Spectrometry Lactobacillus / chemistry Lactobacillus plantarum / chemistry Lauric Acids / isolation & purification Metabolomics / methods Oryza / microbiology Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
12-Hydroxydodecanoic acid Aantifungal activity Fatty acids GC–MS HPLC Lactic acid bacteria Organic acids

Journal

Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234

Informations de publication

Date de publication:
Mar 2020
Historique:
received: 20 10 2019
accepted: 24 01 2020
pubmed: 2 2 2020
medline: 11 11 2020
entrez: 2 2 2020
Statut: ppublish

Résumé

A recent spike in demand for chemical preservative free food has derived the scientific community to develop natural ways of food preservation. Therefore, bio-preservation could be considered as the great alternative over chemical ones owing to its potential to increase shelf-life and nutritional values of foodstuffs. In the present study, lactic acid producing bacterial species were isolated from rice rinsed water and identified by 16S rRNA gene sequencing as Lactobacillus plantarum BCH-1 (KX388380) and Lactobacillus coryniformis BCH-4 (KX388387). Antifungal metabolites from both Lactobacillus species were extracted by polarity-based solvents in which ethyl acetate showed remarkable antifungal activity against Aspergillus flavus and Aspergillus fumigatus by disc diffusion assay. Different organic acids and fatty acids have been identified by reversed-phase high-performance liquid chromatography (RP-HPLC) and gas chromatography-mass spectrometry (GC-MS) analysis, respectively. Lactic acid and citric acid were the major organic acids found in ethyl acetate fractions of L. plantarum and L. coryniformis, respectively. Similarly, 9,12-otadecadienoic acid (Z,Z)-methyl ester and hexadecanoic acid, methyl ester were the major fatty acids found in n-hexane fractions of L. plantarum and L. coryniformis respectively. Moreover, the isolation of novel antifungal metabolites from locally isolated Lactobacillus species was focused and it was revealed that organic acids are important contributors towards antifungal potential. A novel fatty acid (i.e. 12-hydroxydodecanoic acid) has also been explored and found as potential metabolite against filamentous fungi. Conclusively, various metabolites isolated from non-dairy source showed antifungal activity especially against Aspergillus species. Hence, these metabolites have been considered as a good choice for bio-preservation.

Identifiants

DOI: 10.1007/s11033-020-05281-1 PMID: 32006197
pubmed: 32006197
doi: 10.1007/s11033-020-05281-1
pii: 10.1007/s11033-020-05281-1
doi:

Substances chimiques

Acetates 0
Antifungal Agents 0
DNA, Fungal 0
DNA, Ribosomal 0
Lauric Acids 0
RNA, Ribosomal, 16S 0
12-hydroxydodecanoic acid 505-95-3
ethyl acetate 76845O8NMZ

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1871-1881

Subventions

Organisme : Higher Education Commission, Pakistan
ID : 21-432/SRGP/H&D/2014

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Auteurs

Shazia Anwer Bukhari (SA)

Department of Biochemistry, Government College University, Faisalabad, Pakistan.

Mahwish Salman (M)

Department of Biochemistry, Government College University, Faisalabad, Pakistan.

Muhammad Numan (M)

Department of Biochemistry, Government College University, Faisalabad, Pakistan.

Muhammad Rizwan Javed (MR)

Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan.

Muhammad Zubair (M)

Department of Chemistry, Faculty of Sciences, University of Gujrat, Gujrat, Pakistan.

Ghulam Mustafa (G)

Department of Biochemistry, Government College University, Faisalabad, Pakistan. gmustafa_uaf@yahoo.com.
ORCID: 0000-0001-6510-6496

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Classifications MeSH

questionsmedicales.fr › Lipides › Acides gras › Acides gras volatils › Acétates › Characterization of antifungal metabolites...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antifongiques › Characterization of antifungal metabolites...
questionsmedicales.fr › Eucaryotes › Champignons › Deuteromycota › Aspergillus › Characterization of antifungal metabolites...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase inverse › Characterization of antifungal metabolites...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN fongique › Characterization of antifungal metabolites...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN ribosomique › Characterization of antifungal metabolites...
questionsmedicales.fr › Technologie, industrie et agriculture › Industrie › Industrie alimentaire › Technologie alimentaire › Conservation aliments › Characterization of antifungal metabolites...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Chromatographie gazeuse-spectrométrie de masse › Characterization of antifungal metabolites...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Lactobacillaceae › Lactobacillus › Characterization of antifungal metabolites...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus plantarum › Characterization of antifungal metabolites...
questionsmedicales.fr › Lipides › Acides gras › Acides lauriques › Characterization of antifungal metabolites...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Métabolomique › Characterization of antifungal metabolites...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Oryza › Characterization of antifungal metabolites...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Characterization of antifungal metabolites...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Characterization of antifungal metabolites...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Characterization of antifungal metabolites...