Improved bacterial RNA-seq by Cas9-based depletion of ribosomal RNA reads.
Bacteria
/ genetics
CRISPR-Cas Systems
/ genetics
DNA, Complementary
/ genetics
Gene Expression Profiling
/ methods
Gene Library
High-Throughput Nucleotide Sequencing
/ methods
Nucleic Acid Hybridization
/ methods
RNA, Bacterial
/ genetics
RNA, Ribosomal
/ genetics
RNA-Seq
/ methods
Sequence Analysis, RNA
/ methods
Bacteroides
CRISPR
Cas9
DASH
Salmonella
bacterial RNA-seq
ribosomal RNA
Journal
RNA (New York, N.Y.)
ISSN: 1469-9001
Titre abrégé: RNA
Pays: United States
ID NLM: 9509184
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
18
04
2020
accepted:
01
05
2020
pubmed:
30
4
2020
medline:
6
10
2020
entrez:
30
4
2020
Statut:
ppublish
Résumé
A major challenge for RNA-seq analysis of gene expression is to achieve sufficient coverage of informative nonribosomal transcripts. In eukaryotic samples, this is typically achieved by selective oligo(dT)-priming of messenger RNAs to exclude ribosomal RNA (rRNA) during cDNA synthesis. However, this strategy is not compatible with prokaryotes in which functional transcripts are generally not polyadenylated. To overcome this, we adopted DASH (
Identifiants
pubmed: 32345633
pii: rna.075945.120
doi: 10.1261/rna.075945.120
pmc: PMC7373992
doi:
Substances chimiques
DNA, Complementary
0
RNA, Bacterial
0
RNA, Ribosomal
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1069-1078Informations de copyright
© 2020 Prezza et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
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