Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate
Biotechnology
Citrates
Cladosporium
/ enzymology
DNA
/ analysis
Detergents
/ chemistry
Fermentation
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Ions
Iron
/ chemistry
Lactose
/ chemistry
Microscopy, Electron, Scanning
Phylogeny
Polyethylene Glycols
/ chemistry
Polymerase Chain Reaction
Prebiotics
Sequence Analysis, DNA
Temperature
Water
/ chemistry
beta-Galactosidase
/ chemistry
aqueous two-phase system
fungi
phylogenesis
production
β-Galactosidase
Journal
Preparative biochemistry & biotechnology
ISSN: 1532-2297
Titre abrégé: Prep Biochem Biotechnol
Pays: England
ID NLM: 9607037
Informations de publication
Date de publication:
2021
2021
Historique:
pubmed:
11
9
2020
medline:
20
7
2021
entrez:
10
9
2020
Statut:
ppublish
Résumé
β-Galactosidase production, partial purification and characterization by a new fungal were investigated. Partial purification was performed by aqueous two-phase system (ATPS) using polyethylene glycol (PEG) molar mass, PEG concentration, citrate concentration and pH as the independent variables. Purification factor (
Identifiants
pubmed: 32907464
doi: 10.1080/10826068.2020.1815054
doi:
Substances chimiques
Citrates
0
Detergents
0
Ions
0
Prebiotics
0
Water
059QF0KO0R
Polyethylene Glycols
3WJQ0SDW1A
DNA
9007-49-2
Iron
E1UOL152H7
beta-Galactosidase
EC 3.2.1.23
Lactose
J2B2A4N98G
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM