Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense.


Journal

Anaerobe
ISSN: 1095-8274
Titre abrégé: Anaerobe
Pays: England
ID NLM: 9505216

Informations de publication

Date de publication:
Dec 2020
Historique:
received: 06 08 2020
revised: 17 09 2020
accepted: 01 10 2020
pubmed: 16 10 2020
medline: 14 8 2021
entrez: 15 10 2020
Statut: ppublish

Résumé

Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions of the plasmid did not. A phylogenetic study suggested that pCAG had a unique evolutionary history compared with other clostridial bont-harboring plasmids. This suggests that pCAG is possibly a novel type of plasmid expressing the bont/G gene in C. argentinense.

Identifiants

pubmed: 33059044
pii: S1075-9964(20)30137-2
doi: 10.1016/j.anaerobe.2020.102281
pii:
doi:

Substances chimiques

DNA, Bacterial 0
RNA, Ribosomal, 16S 0
botulinum toxin type G 2C3DD06QGJ
Botulinum Toxins EC 3.4.24.69

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102281

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors have no conflicts of interest relevant to this article.

Auteurs

Yoshihiko Sakaguchi (Y)

Department of Microbiology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan. Electronic address: ysakaguchi@med.kitasato-u.ac.jp.

Jumpei Uchiyama (J)

Laboratory of Veterinary Microbiology I, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.

Akira Také (A)

Department of Microbiology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.

Kazuyoshi Gotoh (K)

Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Okayama, Japan.

Masakiyo Sakaguchi (M)

Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Okayama, Japan.

Tomonori Suzuki (T)

Department of Molecular Microbiology, Faculty of Life Science, Tokyo University of Agriculture, Tokyo, Japan.

Yumiko Yamamoto (Y)

Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Okayama, Japan.

Koji Hosomi (K)

Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research, and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health, and Nutrition, Ibaraki, Osaka, Japan.

Tomoko Kohda (T)

Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Masafumi Mukamoto (M)

Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Shunji Kozaki (S)

Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Shunji Hayashi (S)

Department of Microbiology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.

Keiji Oguma (K)

Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Okayama, Japan.

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Classifications MeSH