DCT4-A New Member of the Dicarboxylate Transporter Family in C4 Grasses.
C4 photosynthesis
DCT4
grass evolution
new transporter gene
Journal
Genome biology and evolution
ISSN: 1759-6653
Titre abrégé: Genome Biol Evol
Pays: England
ID NLM: 101509707
Informations de publication
Date de publication:
03 02 2021
03 02 2021
Historique:
accepted:
24
11
2020
entrez:
15
2
2021
pubmed:
16
2
2021
medline:
24
12
2021
Statut:
ppublish
Résumé
Malate transport shuttles atmospheric carbon into the Calvin-Benson cycle during NADP-ME C4 photosynthesis. Previous characterizations of several plant dicarboxylate transporters (DCT) showed that they efficiently exchange malate across membranes. Here, we identify and characterize a previously unknown member of the DCT family, DCT4, in Sorghum bicolor. We show that SbDCT4 exchanges malate across membranes and its expression pattern is consistent with a role in malate transport during C4 photosynthesis. SbDCT4 is not syntenic to the characterized photosynthetic gene ZmDCT2, and an ortholog is not detectable in the maize reference genome. We found that the expression patterns of DCT family genes in the leaves of Zea mays, and S. bicolor varied by cell type. Our results suggest that subfunctionalization, of members of the DCT family, for the transport of malate into the bundle sheath plastids, occurred during the process of independent recurrent evolution of C4 photosynthesis in grasses of the PACMAD clade. We also show that this subfunctionalization is lineage independent. Our results challenge the dogma that key C4 genes must be orthologues of one another among C4 species, and shed new light on the evolution of C4 photosynthesis.
Identifiants
pubmed: 33587128
pii: 6126432
doi: 10.1093/gbe/evaa251
pmc: PMC7883667
pii:
doi:
Substances chimiques
Dicarboxylic Acid Transporters
0
Malates
0
Plant Proteins
0
malic acid
817L1N4CKP
Types de publication
Letter
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
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