Complete genome sequence of a novel citrus virus with characteristics of members of the family Tymoviridae.


Journal

Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870

Informations de publication

Date de publication:
Jul 2021
Historique:
received: 12 07 2020
accepted: 17 03 2021
pubmed: 6 5 2021
medline: 23 6 2021
entrez: 5 5 2021
Statut: ppublish

Résumé

A novel positive-stranded RNA virus provisionally named "citrus virus C" (CVC) was discovered in citrus trees displaying mottling symptoms. Its genome comprises 7,215 nucleotides (nt), excluding the 3' poly(A) tail, and contains two open reading frames (ORFs) that encode a replication-associated polyprotein (RP) and a putative coat protein (CP). The CVC genome contains a 16-nt 'marafibox', which is highly conserved in most viruses belonging to the genus Marafivirus of the same family. Sequence analysis suggested that the virus is most closely related to grapevine Red Globe virus (GRGV), which is yet to be officially classified in the family Tymoviridae. The sequence identities between CVC and GRGV in the whole genome (50.7%, nt) and CP (49.4% for amino acid, and 53.9% for nt) are lower than the thresholds (80% in the genome and 90% in the CP) for species demarcation in the family. Therefore, it is legitimate to propose that CVC is a member of new species in the family Tymoviridae.

Identifiants

pubmed: 33950289
doi: 10.1007/s00705-021-05082-2
pii: 10.1007/s00705-021-05082-2
doi:

Substances chimiques

RNA, Viral 0
Viral Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2055-2058

Subventions

Organisme : National Key R&D Program of China
ID : 2019YFD1001800
Organisme : National Natural Science Foundation of China
ID : 32072389
Organisme : Innovation Program for Chongqing's Overseas Returnees
ID : cx2019013
Organisme : Overseas Expertise Introduction Project for Discipline Innovation (111 Center)
ID : B18044

Références

Hily J-M, Candresse T, Garcia S, Vigne E, Tannière M, Komar V, Barnabé G, Alliaume A, Gilg S, Hommay G (2018) High-throughput sequencing and the viromic study of grapevine leaves: from the detection of grapevine-infecting viruses to the description of a new environmental Tymovirales member. Front Microbiol 9:1782
doi: 10.3389/fmicb.2018.01782
Wang L, Lv X, Zhai Y, Fu S, Wang D, Rayner S, Tang Q, Liang G (2012) Genomic characterization of a novel virus of the family Tymoviridae isolated from mosquitoes. PLoS One 7:e39845
doi: 10.1371/journal.pone.0039845
Katsuma S, Tanaka S, Omuro N, Takabuchi L, Daimon T, Imanishi S, Yamashita S, Iwanaga M, Mita K, Maeda S (2005) Novel macula-like virus identified in Bombyx mori cultured cells. J Virol 79:5577–5584
doi: 10.1128/JVI.79.9.5577-5584.2005
Dreher TW, Edwards MC, Gibbs AJ, Haenni A-L, Hammond RW, Jupin I, Koenig R, Sabanadzovic S, Martelli GP (2012) Family Tymoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier Academic Press, San Diego, pp 944–952
Martelli G, Sabanadzovic S, Abou Ghanem-Sabanadzovic N, Edwards M, Dreher T (2002) The family Tymoviridae. Adv Virol 147:1837–1846
Ding S, Howe J, Keese P, Mackenzie A, Meek D, Osorlo-Keese M, Skotnicki M, Srifah P, Torronen M, Gibbs A (1990) The tymobox, a sequence shared by most tymoviruses: its use in molecular studies of tymoviruses. Nucleic Acids Res 18:1181–1187
doi: 10.1093/nar/18.5.1181
Edwards MC, Weiland JJ (2014) Coat protein expression strategy of oat blue dwarf virus. Virology 450:290–296
doi: 10.1016/j.virol.2013.12.018
Izadpanah K, Zhang YP, Daubert S, Masumi M, Rowhani A (2002) Sequence of the coat protein gene of bermuda grass etched-line virus, and of the adjacent ‘marafibox’motif. Virus Genes 24:131–134
doi: 10.1023/A:1014516515454
Hammond R, Ramirez P (2001) Molecular characterization of the genome of maize rayado fino virus, the type member of the genus Marafivirus. Virology 282:338–347
doi: 10.1006/viro.2001.0859
Igori D, Lim S, Baek D, Seo E, Cho I-S, Choi G-S, Lim H-S, Moon JS (2017) Complete nucleotide sequence and genome organization of peach virus D, a putative new member of the genus Marafivirus. Adv Virol 162:1769–1772
Sabanadzovic S, Abou Ghanem-Sabanadzovic N, Saldarelli P, Martelli GP (2001) Complete nucleotide sequence and genome organization of grapevine fleck virus. J Gen Virol 82:2009–2015
doi: 10.1099/0022-1317-82-8-2009
Martelli G, Sabanadzovic S, Abou Ghanem-Sabanadzovic N, Saldarelli P (2002) Maculavirus, a new genus of plant viruses. Adv Virol 147:1847–1853
Bradel BG, Preil W, Jeske H (2000) Sequence analysis and genome organisation of poinsettia mosaic virus (PnMV) reveal closer relationship to marafiviruses than to tymoviruses. Virology 271:289–297
doi: 10.1006/viro.2000.0335
Xu Q, Chen LL, Ruan X, Chen D, Zhu A, Chen C, Bertrand D, Jiao WB, Hao BH, Lyon MP, Chen J, Gao S, Xing F, Lan H, Chang JW, Ge X, Lei Y, Hu Q, Miao Y, Wang L, Xiao S, Biswas MK, Zeng W, Guo F, Cao H, Yang X, Xu XW, Cheng YJ, Xu J, Liu JH, Luo OJ, Tang Z, Guo WW, Kuang H, Zhang HY, Roose ML, Nagarajan N, Deng XX, Ruan Y (2013) The draft genome of sweet orange (Citrus sinensis). Nat Genet 45:59–66
doi: 10.1038/ng.2472
Cretazzo E, Velasco L (2017) High-throughput sequencing allowed the completion of the genome of grapevine Red Globe virus and revealed recurring co-infection with other tymoviruses in grapevine. Plant Pathol 66:1202–1213
doi: 10.1111/ppa.12669
Cao M, Wu Q, Atta S, Su H, Yu Y, Chen H, Zhou C (2016) First molecular evidence of citrus yellow vein clearing virus from citrus in Punjab, Pakistan. Plant Dis 100:540–540
doi: 10.1094/PDIS-05-15-0609-PDN
Cao M, Yu Y-Q, Tian X, Yang F, Li R, Zhou C (2017) First report of citrus leaf blotch virus in lemon in China. Plant Dis 101:1561–1561
doi: 10.1094/PDIS-10-16-1500-PDN
Alabdullah A, Minafra A, Elbeaino T, Saponari M, Savino V, Martelli GP (2010) Complete nucleotide sequence and genome organization of olive latent virus 3, a new putative member of the family Tymoviridae. Virus Res 152:10–18
doi: 10.1016/j.virusres.2010.05.010
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549
doi: 10.1093/molbev/msy096
Maccheroni W, Alegria MC, Greggio CC, Piazza JP, Kamla RF, Zacharias PRA, Barjoseph M, Kitajima EW, Assumpção LC, Camarotte G (2005) Identification and genomic characterization of a new virus (Tymoviridae family) associated with citrus sudden death disease. J Virol 79:3028–3037
doi: 10.1128/JVI.79.5.3028-3037.2005
Cui T, Bin Y, Yan J, Mei P, Li Z, Zhou C, Song Z (2018) Development of infectious cDNA clones of citrus yellow vein clearing virus using a novel and rapid strategy. Phytopathology 108:1212–1218
doi: 10.1094/PHYTO-02-18-0029-R

Auteurs

Liu Yang (L)

National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Song Zhang (S)

National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Shiqiang Mei (S)

National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Qiyan Liu (Q)

National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Yan Zhou (Y)

National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Ruhui Li (R)

National Germplasm Resources Laboratory, USDA-ARS, Beltsville, MD, 20705, USA.

Mengji Cao (M)

National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China. caomengji@cric.cn.
Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China. caomengji@cric.cn.

Articles similaires

Genome, Chloroplast Phylogeny Genetic Markers Base Composition High-Throughput Nucleotide Sequencing
Animals Hemiptera Insect Proteins Phylogeny Insecticides
Amaryllidaceae Alkaloids Lycoris NADPH-Ferrihemoprotein Reductase Gene Expression Regulation, Plant Plant Proteins
Drought Resistance Gene Expression Profiling Gene Expression Regulation, Plant Gossypium Multigene Family

Classifications MeSH