First report of mitochondrial COI in foraminifera and implications for DNA barcoding.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
12 11 2021
12 11 2021
Historique:
received:
24
06
2021
accepted:
28
10
2021
entrez:
13
11
2021
pubmed:
14
11
2021
medline:
27
1
2022
Statut:
epublish
Résumé
Foraminifera are a species-rich phylum of rhizarian protists that are highly abundant in many marine environments and play a major role in global carbon cycling. Species recognition in Foraminifera is mainly based on morphological characters and nuclear 18S ribosomal RNA barcoding. The 18S rRNA contains variable sequence regions that allow for the identification of most foraminiferal species. Still, some species show limited variability, while others contain high levels of intragenomic polymorphisms, thereby complicating species identification. The use of additional, easily obtainable molecular markers other than 18S rRNA will enable more detailed investigation of evolutionary history, population genetics and speciation in Foraminifera. Here we present the first mitochondrial cytochrome c oxidase subunit 1 (COI) gene sequences ("barcodes") of Foraminifera. We applied shotgun sequencing to single foraminiferal specimens, assembled COI, and developed primers that allow amplification of COI in a wide range of foraminiferal species. We obtained COI sequences of 49 specimens from 17 species from the orders Rotaliida and Miliolida. Phylogenetic analysis showed that the COI tree is largely congruent with previously published 18S rRNA phylogenies. Furthermore, species delimitation with ASAP and ABGD algorithms showed that foraminiferal species can be identified based on COI barcodes.
Identifiants
pubmed: 34772985
doi: 10.1038/s41598-021-01589-5
pii: 10.1038/s41598-021-01589-5
pmc: PMC8589990
doi:
Substances chimiques
RNA, Ribosomal, 18S
0
Electron Transport Complex IV
EC 1.9.3.1
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
22165Subventions
Organisme : National Science Foundation
ID : DBI-2119963
Informations de copyright
© 2021. The Author(s).
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