Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece.


Journal

Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870

Informations de publication

Date de publication:
01 Jun 2023
Historique:
received: 07 02 2023
accepted: 25 04 2023
medline: 5 6 2023
pubmed: 1 6 2023
entrez: 1 6 2023
Statut: epublish

Résumé

Using high-throughput sequencing, we identified a novel carlavirus sequence in a 28-year-old 'Kotsifali' grapevine sample collected in Heraklion (Crete, Greece). Using RT-PCR and 5'/3' RACE together with Sanger sequencing, the complete genome sequence of 8299 nt was confirmed and found to contain five open reading frames (ORFs) but to lack an ORF6, which is present in some members of the genus Carlavirus. The novel sequence is most similar to those of two carlaviruses infecting caper, and taking into account the ICTV nomenclature, we propose the name "grapevine carlavirus 1" for this new virus.

Identifiants

pubmed: 37261601
doi: 10.1007/s00705-023-05795-6
pii: 10.1007/s00705-023-05795-6
pmc: PMC10235145
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

172

Informations de copyright

© 2023. The Author(s).

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Auteurs

Konstantina Katsarou (K)

Department of Biology, University of Crete, Vassilika Vouton, Heraklion/Crete, GR-71409, Greece. katsarou@imbb.forth.gr.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion/Crete, GR-71110, Greece. katsarou@imbb.forth.gr.

Christos Andronis (C)

Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion/Crete, GR-71110, Greece.

Anthony James (A)

Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion/Crete, GR-71110, Greece.

Kostas Euthymiou (K)

Department of Biology, University of Crete, Vassilika Vouton, Heraklion/Crete, GR-71409, Greece.

Nikoleta Kryovrysanaki (N)

Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion/Crete, GR-71110, Greece.

Polykseni G Pappi (PG)

Laboratory of Plant Virology, Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, Institute of Olive Tree, Subtropical Crops and Viticulture, Hellenic Agricultural Organization - "DIMITRA", 71307, Heraklion, Crete, GR, Greece.

Kriton Kalantidis (K)

Department of Biology, University of Crete, Vassilika Vouton, Heraklion/Crete, GR-71409, Greece. kalantidis@imbb.forth.gr.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion/Crete, GR-71110, Greece. kalantidis@imbb.forth.gr.

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Classifications MeSH